LOCUS       sequence-158322-       12011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LexA-GAD under the control of 57F03 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Yapici N, Cohn R, Schusterreiter C, Ruta V, Vosshall LB
  TITLE     A Taste Circuit that Regulates Ingestion by Integrating Food and 
            Hunger Signals.
  JOURNAL   Cell. 2016 Apr 21;165(3):715-29. doi: 10.1016/j.cell.2016.02.061. 
            Epub 2016 Mar 31.
  PUBMED    27040496
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.061"; journalName: "Cell"; date: 
            "2016-04-21- 21"; volume: "165"; issue: "3"; pages: "715-29"
COMMENT     Sequence Label: 57F03_LexA-GAD
FEATURES             Location/Qualifiers
     source          1..12011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2174..2198)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2394..2414
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2424..2675,2676..3029)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     complement(5506..5522)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5506..5522)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(6533..7147,7218..7349,7553..7868,7930..8584,
                     8659..8932,9334..9405))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9857..9875
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    10100..10169
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(10256..10844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10336..10355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11015..11806,11807..11875))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11638..11657
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11876..11980)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gcaggctccg aattcgccct
      121 tcaaggaacg agttgcgaaa agaggctgtt agaataagtg ggatgttcaa atctaaccaa
      181 ccaatatatg atatatttta attcatagat gtaagaagtt ttcagaaaag ttttaaaaga
      241 aatcgttgga tttaggtata agggaagcaa gactctcccg ataagaaaga acggaacttt
      301 tggaatatat attcttttga agaattttac tttacttaat caacttaaaa catttaataa
      361 atgtttgcgt gtgttatatg ctaatcgaaa cgatggtaga ggacttaaca taagatgcgt
      421 tctacgtata aaagatattc agcttctttt gaagaaatct aataaatact aaataaatgg
      481 ataaaacttt caattgtttt tttgaagttt tttttttatt gttttttgat taaatatagt
      541 gccggtggtg ccctacctaa ctttgcagtt gacaacttcc gtggcggtgc agcacataag
      601 ctttcatcct tcacccaaat caccgaccga cgttgattga tgggttcgat tcatttcccc
      661 cagttcgaca aacagtgggt agaaagcttt gcagctcccc gctgcacatg aaaagctttt
      721 caagcgggga aaaccattcg actcgtgcca aattccagag acattgcacg cactttgaca
      781 cccatcaaga ggcgtgtgcc gggttttggc caataactgg gcaggggggc ggctatatct
      841 ggccatatcc aggtggggag gggtcggcac acttagcgtt tgccggtgct aaacgagacg
      901 aaacgaaaca aatgaatgaa aatattatgt ggccatagaa aatgtcactc gtcgacggcg
      961 tgaagcaact aaaaagcccg aactgtcttg agaatcgcga ggcgcattcg gccaagttgg
     1021 tggttctacc atggcctctt gtgttgttgt tggcgcacag tggctaatgt cccgagccaa
     1081 cgttgctgct ccatagacac tatgaaatat tgttgccaaa atacacacac gaatgcggcc
     1141 cgagacggac ccgtaaataa gactggaagg tgcgcgaaat ttcgaatggt ggtgtcacta
     1201 agttgggcta agttgacgcc ctgtgcatca caagcttaca caaaatatag tttaattaat
     1261 ctaaattaga tttgaattgt aattattatt atttgtttac atttcaatat actttagtaa
     1321 gataaacaaa gaacaatatt cttacttata agctaacaag taagctggct ggacattact
     1381 catacgcacg gtggccttcg gtcatttggt attattgcat catctaatat tattgcatct
     1441 tattttggct tttaattcca agatgtgttg tttacattat cgtggtttat ttaggattat
     1501 gtgcatcaca aagtcaaaat tcccaactaa gtgttttata aatgggccaa agcaaacatt
     1561 attacaagtt tttgttaatt agccttttta attacccaaa aattgcgttg tattcggagc
     1621 cattgtgctt agtctctaac atcggctagt cccatcaaga tatcgccgct tttcaagatt
     1681 taattgagcc ggcacgttcg ctggacgctc ggtcattaca accatgtcgc tcaccttccg
     1741 cgttcatgtg agggaaaaac agcgacgctt cgtcgctggc tttcagatac agatacaaat
     1801 ctgaatgaaa tgtcgccgcg gcttatcaga taagtttgga gcgcgctgga gtgcggttgc
     1861 aggctgctct cgggaatcag aattcgaatc gaaatcaaat taactcgata acttattgaa
     1921 gagaagtcgg gccccctaga aaaacagccg ggaatacgga atactgaata ctgaatacgg
     1981 tatacggatc tcgacctcgt cggtaatttg gtgcgtttgc gtgccagtcg agtatctgaa
     2041 tcttagaatc gagtatctag cattgcgaag cgcgagtcca gacctgtccc tcctccgccc
     2101 ggtcgcctcc aataaattat tataattaag catagttgag gggacatcga gtcgcagccg
     2161 aagggcgaat tcgacccagc tttcttgtac aaagtggtga taaacggccg gccgagctcg
     2221 cccggggatc gagcgcagcg gtataaaagg gcgcggggtg gctgagagca tcagttgtga
     2281 atgaatgttc gagccgagca gacgtgccgc tgccttcgtt aatatccttt gaataagcca
     2341 actttgaatc acaagacgca taccaaacgg taccgctagc aatcaaaatg ccacccaaga
     2401 agaagcgaaa agtagaagat ccaatgaagg ctctcacggc ccgacaacag gaagtttttg
     2461 atttgatacg ggatcatata tcccaaacgg gtatgcctcc gacccgcgca gagatagcac
     2521 agcgactggg ctttcgatcg cctaacgccg cggaggagca cttgaaggca ctggcccgca
     2581 agggtgtcat tgaaatcgtg tccggtgcga gccgcggaat ccggctgttg caggaggagg
     2641 aagagggcct gccactggtg ggacgcgtgg ccgctggcga gccgctgctg gcccagcaac
     2701 acatagaggg acactatcag gtggacccct ccttgtttaa gccaaatgct gatttcctgt
     2761 tgcgggtgtc gggaatgtcc atgaaggaca tcggtattat ggatggtgac ctcctcgccg
     2821 tccataagac acaggatgtc aggaacggcc aggtagtcgt tgccaggata gacgatgagg
     2881 tcactgtgaa acgtctcaag aagcaaggca ataaggtcga gctgctgccg gagaatagcg
     2941 agttcaagcc gatcgtggtg gatctgcgac agcagtcctt tactatcgag ggcttggccg
     3001 tgggtgtgat ccgcaacgga gattggctgg gatccatcga cagtgcggcc caccacgaca
     3061 acagcaccat tccactggac ttcatgcctc gtgacgcact gcatggattt gactggagcg
     3121 aggaggatga tatgagcgac ggactcccat tcctgaagac cgaccccaat aacaatggtt
     3181 tctttggcga cggcagcctg ctgtgtatcc tgcgaagtat tggttttaag ccggaaaatt
     3241 acacgaacag taatgtcaat cgtcttccca caatgatcac cgaccgttac acgctggcca
     3301 gtcgcagtac tacaagccgc ctgctgcaaa gctatcttaa caacttccac ccctactgcc
     3361 ccatagtgca tagtcctact ctcatgatgc tttacaacaa tcagattgag atagcatcga
     3421 aggaccagtg gcagatcctc ttcaactgca ttctcgccat cggcgcttgg tgtattgaag
     3481 gcgagagcac agatatcgac gtgttttact atcaaaacgc caagtcgcac ctcacctcga
     3541 aggtcttcga aagtggaagc attatcctcg tgacagccct ccatctgctg agtcgttata
     3601 cccagtggcg tcagaaaact aatacatcct acaactttca ttcgttcagc attcggatgg
     3661 cgattagcct gggacttaat cgtgacctcc caagtagctt cagcgattcg tcgatcctgg
     3721 aacagcgccg gaggatttgg tggagtgtgt acagctggga gatccagctg tccctcttgt
     3781 acggacgcag cattcagctg agtcagaaca cgatttcctt cccgtccagc gtggacgacg
     3841 ttcaacggac aactacgggc ccgaccatct accacggtat cattgagacg gcccgcttgc
     3901 ttcaagtgtt tacgaagatt tatgagctgg ataagacagt gaccgcggag aagagtccga
     3961 tctgcgccaa gaaatgcctg atgatctgca atgagataga ggaggtgagc cgccaggcac
     4021 ccaagttcct gcagatggac ataagcacta cggcgctgac taatctcctc aaagaacacc
     4081 cctggctgag tttcacccgc ttcgagctga agtggaaaca gctgagtctg attatatacg
     4141 ttttgcggga ctttttcaca aattttacgc agaagaagtc gcagcttgag caagatcaga
     4201 acgaccatca gtcctacgag gtaaagaggt gcagcataat gctctccgac gcagcccagc
     4261 gcaccgtgat gtcggtgtcg tcctatatgg acaaccataa tgtgacccct tatttcgcct
     4321 ggaattgctc ctactatttg ttcaacgccg tgttggtccc aatcaagact cttctgagca
     4381 actccaaatc gaacgccgag aataacgaga cagcacagct cttgcagcaa attaacaccg
     4441 tgttgatgct gttgaagaaa ctcgctacat tcaagattca gacttgcgaa aagtacatcc
     4501 aggtgttgga agaggtgtgc gccccattcc ttctttcgca atgcgcaatt cccctcccgc
     4561 acataagtta caataattcc aacggctccg ctatcaagaa tatcgtcggc tccgctacaa
     4621 tcgcgcagta tcccacgctg cctgaggaaa atgttaacaa catatcggtg aagtatgtaa
     4681 gtccaggcag cgtcggcccg agccctgtgc cgctgaaaag cggagcctcc ttcagcgatc
     4741 tggtcaagct gctcagcaac cgcccaccat cccgcaactc ccctgtcact ataccccgat
     4801 cgaccccgtc gcatcgtagc gttaccccat tcctgggaca acagcagcaa ctgcagagtc
     4861 tggtgccact tacgccctcg gcattgtttg gcggagccaa cttcaaccag agtggtaaca
     4921 ttgcggacag ctccttgagc ttcacgttta ccaacagctc caacggtcca aacttgatca
     4981 cgactcagac aaactcccag gcattgtccc agcccattgc cagctcgaat gtgcatgata
     5041 actttatgaa taatgaaatt actgcctcca agattgacga cggcaacaac agcaagcccc
     5101 tttccccagg ctggactgac cagacggcct acaatgcttt cggtatcacc actggcatgt
     5161 tcaacaccac aacgatggac gacgtatata actatctttt tgatgatgag gatactccac
     5221 cgaacccaaa gaaggagtag gtttaaacaa gcttatcgat accgtcgact aaagccaaat
     5281 agaaaattat tcagttcctg gcttaagttt ttaaaagtga tattatttat ttggttgtaa
     5341 ccaaccaaaa gaatgtaaat aactaataca taattatgtt agttttaagt tagcaacaaa
     5401 ttgattttag ctatattagc tacttggtta ataaatagaa tatatttatt taaagataat
     5461 tgcgttttta ttgtcaggga gtgagtttgc ttaaaaactc gtttagatcc actagttcta
     5521 gagcggccgc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
     5581 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
     5641 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
     5701 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggaggcg
     5761 cgccctagtt ccagtgaaat ccaagcattt tctaaattaa atgtattctt attattatag
     5821 ttgttatttt tgatatatat aaacaacact attatgccca ccattttttt gagatgcatc
     5881 tacacaagga acaaacactg gatgtcactt tcagttcaaa ttgtaacgct aatcactccg
     5941 aacaggtcac aaaaaattac cttaaaaagt cataatatta aattagaata aatatagctg
     6001 tgagggaaat atatacaaat atattggagc aaataaattg tacatacaaa tatttattac
     6061 taatttctat tgagacgaaa tgaaccactc ggaaccattt gagcgaaccg aatcgcgcgg
     6121 aactaacgac agtcgctcca aggtcgtcga acaaaaggtg aatgtgttgc ggagagcggg
     6181 tgggagacag cgaaagagca actacgaaac gtggtgtggt ggaggtgaat tatgaagagg
     6241 gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc ggtgttttat gtagcgataa
     6301 acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt tggttagaag acaaatccaa
     6361 agtctacttg tggggatgtt cgaaggggaa atacttgtat tctataggtc atatcttgtt
     6421 tttattggca caaatataat tacattagct ttttgagggg gcaataaaca gtaaacacga
     6481 tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg gatatatgtc ggctactcct
     6541 tgcgtcgggc ccgaagtctt agagccagat atgcgagcac ccggaagctc acgatgagaa
     6601 tggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc gtctccagga
     6661 tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct
     6721 ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac catgagaggt
     6781 acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag ccgccaaaga
     6841 gcaggaatgg tatgataacc ggcggaccca cagacagcgc catcgaggtc gaggagctgg
     6901 cgcaggatat tagatatccg aaggacgttg acacattggc caccagagtg accagcgcca
     6961 ggcagttgaa gaagtgcagc actccggccc gcagtccgat catcggatag gcaatcgccg
     7021 tgaagaccag tggcactgtg agaaaaagcg gcaattcggc aatcgttttg cccagaaagt
     7081 atgtgtcaca gcgataaagt cgacttcggg cctccctcat aaaaactggc agctctgagg
     7141 tgaacaccta aatcgaatcg attcattaga aagttagtaa attattgaaa tgcaaatgta
     7201 ttctaaacat gacttacatt tatcgtggca aagacgtttt gaaaggtcat gttggtcagg
     7261 aagaggaaga tggctccgtt gatattcatc acacccactt gcgtgagttg ttggcccaaa
     7321 aagatgaggc caatcaagat ggcaaccatc tgcaaattaa aatgttactc gcatctcatt
     7381 aatattcgcg agttaaatga aatttattta tcttctgcaa aactataaac tatacatctc
     7441 attgaaaaaa actaagaagg gtgtggaatc aggcaattct atctaaaatc tagcgaattt
     7501 gtttccaaga attgtaagcg ttatatcatt tgtttccact ggaaccactc accgttgtct
     7561 gaataagtcg cacttttacg aggagtggtt ccttgagcac cgacagccag gatcgccaca
     7621 ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt gtacccattc tccggctgct
     7681 ccagtggctt ctccagattt ttggtggcca acaactgctc catatcccgg gctactttgc
     7741 taatggcaaa attgtcgcat atcttggcga tccgatcacg ggactcgatc tcccgtccgg
     7801 gcacaacggc caacacctgt acgtaaaagt ccgccggatt gtagttggta ggacactggg
     7861 cacccacgct ggataggagt tgagatgtta tgtaatacta gataccctta ataaacacat
     7921 cgaactcact aggaaaagaa gtcgacggct tcgctgggag tgcccaagaa agctaccctg
     7981 ccctcggcca tcagaaggat cttgtcaaag agctcaaaca gctcggaaga cggctgatga
     8041 atggtcagga tgacggtctt gcccttctgc gacagcttct tcagcacctg gacgacgctg
     8101 tgggcggtaa aggagtccag tccggaggtg ggctcatcgc agatcagaag cggcggatcg
     8161 gttagagcct cggaggcgaa tgccagacgc ttcctttctc cgccggacag acctttcacc
     8221 ctgccgggca caccgatgat cgtgtgctga catttgctga gcgaaagctc ctggatcacc
     8281 tgatccacgc gggccactcg ctgccgatag gtcagatgtc gtggcatccg caccatggct
     8341 tggaaaatca ggtgttccct ggccgttagg gagccgataa agaggtcatc ctgctggaca
     8401 taggcgcacc tggcctgcat ctccttggcg tccacaggtt ggccattgag cagtcgcatc
     8461 ccggatggcg atacttggat gccctgcggc gatcgaaagg caagggcatt cagcagggtc
     8521 gtctttccgg caccggaact gcccatcacg gccaaaagtt cgcccggata ggccacgccg
     8581 caaactgagt ttcaaattgg taattggacc ctttattaag atttcacaca gatcagccga
     8641 ctgcgaatag aaactcaccg ttcttgagca aatgtttcct gggcgccggt atgtgtcgct
     8701 cgttgcagaa tagtccgcgt gtccggttga ccagctgccg ccatccggag cccggctgat
     8761 tgaccgcccc aaagatgtcc atattgtgcc aggcataggt gaggttctcg gctagttggc
     8821 cgctccctga accggagtcc tccggcggac tgggtggccg gagcgtgccg tagtttttgg
     8881 cctgcccgaa gccctggtta atgcagctct gcgaagccgc tccgctgtca ccctgcaatg
     8941 ataggggatc tcaaatatca actactagcg ttatgctcat ctaaccccga acaaaaagta
     9001 ccccgaagta tcctacgaag taggtttata cttttattta ttttttgtgc atctaggatc
     9061 agcttaaaat atctggttgt tatatttttt gtaaaaaaga atatagtcga aaatgaatgc
     9121 ctttagatgt cttgatcatg atatgatctc aaaaattgtc ttatatagcg agaacagcta
     9181 ccagaataat ctgtttcgtg tcactatttg tttgtgcaat tgcggtttgg gatttttgtg
     9241 ggtcgcagtt ctcacgccgc atacaatttg atgttgcaat cgcagttcct atagatcaag
     9301 tgaacttaag atgtatgcac atgtactact cacattgttc agatgctcgg cagatgggtg
     9361 tttgctgcct ccgcgaatta atagctcctg atcctcttgg cccattgccg ggatttttca
     9421 cactttcccc tgcttaccca cccaaaacca atcaccaccc caatcactca aaaaacaaac
     9481 aaaaataaga agcgagagga gttttggcac agcactttgt gtttaattga tggcgtaaac
     9541 cgcttggagc ttcgtcacga aaccgctgac aaaatgcaac tgaaggcgga cattgacgct
     9601 acgtaacgct acaaacggtg gcgaaagaga tagcggacgc agcggcgaaa gagacggcga
     9661 tatttctgtg gacagagaag gaggcaaaca gcgctgactt tgagtggaat gtcattttga
     9721 gtgagaggta atcgaaagaa cctggtacat caaataccct tggatcgaag taaatttaaa
     9781 actgatcaga taagttcaat gatatccagt gcagtaaaaa aaaaaaatgt tttttttatc
     9841 tactttccgc aaaaatgggt tttattaact tacatacata ctaggcgcgc ccatatgttc
     9901 ggcttgtcga catgcccgcc gtgaccgtcg agaacccgct gacgctgccc cgcgtatccg
     9961 cacccgccga cgccgtcgca cgtcccgtgc tcaccgtgac caccgcgccc agcggtttcg
    10021 agggcgaggg cttcccggtg cgccgcgcgt tcgccgggat caactaccgc cacctcgacc
    10081 cgttcatcat gatggaccag atgggtgagg tggagtacgc gcccggggag cccaagggca
    10141 cgccctggca cccgcaccgc ggcttcgaga ccgtgaccta catcgtcgac ggtaacatgt
    10201 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10261 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10321 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10381 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10441 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10501 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10561 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10621 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10681 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    10741 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10801 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    10861 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    10921 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    10981 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11041 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11101 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11161 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11221 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11281 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11341 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11401 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11461 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    11521 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    11581 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    11641 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    11701 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    11761 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    11821 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    11881 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    11941 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12001 cacctgacgt c
//
