LOCUS       sequence-158325-       10554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GAL80 under the control of  Ir25a enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Yapici N, Cohn R, Schusterreiter C, Ruta V, Vosshall LB
  TITLE     A Taste Circuit that Regulates Ingestion by Integrating Food and 
            Hunger Signals.
  JOURNAL   Cell. 2016 Apr 21;165(3):715-29. doi: 10.1016/j.cell.2016.02.061. 
            Epub 2016 Mar 31.
  PUBMED    27040496
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.061"; journalName: "Cell"; date: 
            "2016-04-21- 21"; volume: "165"; issue: "3"; pages: "715-29"
COMMENT     Sequence Label: Ir25a_GAL80
FEATURES             Location/Qualifiers
     source          1..10554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1151..1175)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          1357..1423
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     misc_feature    2751..3339
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2804..2824)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3222..3233)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     intron          3431..3496
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3626..3646
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3918..4039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3955..3974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4009..4028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(5076..5690,5761..5892,6096..6411,6473..7127,
                     7202..7475,7877..7948))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8400..8418
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8643..8712
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8799..9387)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8879..8898)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9558..10349,10350..10418))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10181..10200
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10419..10523)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gcaggctccg aattcgccct
      121 tcgtcaattg ttgttgcttg cttgcctaat ggggagcgct tggccaagcc ttatcagtgc
      181 gatttgtctc gactagatta tagattgtgt gagtcagaag taatctaaaa tagttgctaa
      241 tttcgtttcg tttgatgact gtcacagttt ttaataagga tcacttccta tttttaaatc
      301 agaagttcct ataatagctt tgcccaattt atgaccctat tcccaatgtt tccactccac
      361 caattcgaat aataatttca tgatgatttg acaatataac agctacttag taccacataa
      421 taactccccc ccgaactgaa tcctccttcc ctaaccgcca ccaattaacg cttgagttac
      481 agcgcacgaa caatcgaacc aggatatggg ttcacgcttg gattggggtg ttgctgatgt
      541 ggcgttgtgg gcgattgccg accaaattga ttatcaccaa ggtaacaccc atgtgtccac
      601 acattgttct gcttgaaagt ggaattgtca gtgacagtat aggttgtaca tatctgtatc
      661 ctttttcaaa agcgtagttt cctgtcccag tcaccatttg ttgttcgtcc tgtgcggtcg
      721 attttttagc tcttaaaaac ccgaatcaga aatcatatgt gaaaaacata ataatgattt
      781 tgatgaatcc gaaaacttcg aaaatcctgt ggctgctggg atttctatcg ttattaagca
      841 gctttagttt ggagatcgct gcgcaaacca ctcaaaatat caatgtgtgt aagttgagca
      901 tcgatcgata tgcctaaaga tatatatata tagatgataa atacataatc atatacctaa
      961 tgattatagt ttagtgcact catttaaaga tctttcgaaa ctatatcgaa tattatcgaa
     1021 ctcttttggc tgagtggatt aaaagtaaac taattagatg aaaaccaata tggattggga
     1081 ttactttttt gggtggtgcc agtcataatg agtttttagg gcaaacaaac aaacgcaaag
     1141 ggcgaattcg acccagcttt cttgtacaaa gtggtgataa acggccggcc gagctcgccc
     1201 ggggatcgag cgcagcggta taaaagggcg cggggtggct gagagcatca gttgtgaatg
     1261 aatgttcgag ccgagcagac gtgccgctgc cttcgttaat atcctttgaa taagccaact
     1321 ttgaatcaca agacgcatac caaacggtac caaaaggtag gttcaaccac tgatgcctag
     1381 gcacaccgaa acgactaacc ctaattctta tcctttactt caggctagca atcaaaatgg
     1441 attacaacaa aaggagtagt gtgagtacgg tgccgaatgc tgctcccatt cgcgtgggct
     1501 tcgtgggatt gaacgcggct aagggttggg ccattaaaac gcattatcca gccatactgc
     1561 agctgagctc ccagttccaa ataacagcgc tttattcccc caagatcgag acgtccattg
     1621 cgacaattca gaggctgaag ttgtccaacg ccacagcttt cccaacactc gagagcttcg
     1681 cctcgagctc cacgatcgat atgattgtga tcgcaatcca ggtggcttcc cactacgagg
     1741 tggtaatgcc cctgctcgag ttttcgaaga acaatcctaa tctgaagtat ttgtttgtgg
     1801 aatgggcact ggcctgctcg ctcgatcagg cggagtcgat ctataaggcc gctgcagagc
     1861 ggggcgtgca aacaataatc agtctgcagg gacgaaagag cccgtacatt ttgcgggcaa
     1921 aagagcttat ctcccagggc tacatcggcg atataaatag catcgagatt gcaggtaacg
     1981 gaggttggta cggttacgag cggccggtga aaagcccgaa atacatttac gagatcggaa
     2041 acggagttga tctggtgacc accacgttcg gtcacacgat agatatattg cagtacatga
     2101 ccagttcgta cttcagccgt atcaatgcca tggtgtttaa caacattcca gaacaggagc
     2161 tcattgatga gaggggcaat cgcctgggcc agcgggtccc aaagactgtc ccagatcatc
     2221 tcttgttcca gggcacgctg ctcaacggaa atgttcctgt gtcctgttcc tttaagggag
     2281 gcaagcctac gaaaaagttc accaagaatc tggtcataga tatccatggt acaaagggtg
     2341 atctgaagct ggagggtgac gctggatttg cggaaatctc gaatctcgtt ctgtattatt
     2401 cgggcacccg cgccaatgat tttcccctgg ccaatggtca gcaggcaccc ttggaccccg
     2461 gctacgacgc aggcaaggag attatggagg tgtatcacct ccgaaactac aacgcgatcg
     2521 ttggtaatat acaccggctc taccagagta tcagcgattt ccacttcaac accaagaaaa
     2581 tccccgagct tccatcgcag ttcgtaatgc aaggcttcga tttcgaaggc tttcccaccc
     2641 tgatggatgc cttgatcttg catcgcctga ttgaaagtgt ctacaagtcg aatatgatgg
     2701 gcagtaccct gaatgtttcc aacatctccc actactcgct ttaaaagctt aatcaacctc
     2761 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2821 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2881 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2941 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3001 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     3061 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     3121 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     3181 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     3241 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     3301 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgaagcttcc tagggatctt
     3361 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     3421 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     3481 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     3541 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     3601 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     3661 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     3721 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     3781 aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     3841 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3901 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3961 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4021 aactcatcaa tgtatcttat catgtctacc ggtactagtt ctagagcggc cgctggccac
     4081 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
     4141 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
     4201 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
     4261 aaacgcggaa gtcagcgccc tgcaccatta tgttccggag gcgcgcccta gttccagtga
     4321 aatccaagca ttttctaaat taaatgtatt cttattatta tagttgttat ttttgatata
     4381 tataaacaac actattatgc ccaccatttt tttgagatgc atctacacaa ggaacaaaca
     4441 ctggatgtca ctttcagttc aaattgtaac gctaatcact ccgaacaggt cacaaaaaat
     4501 taccttaaaa agtcataata ttaaattaga ataaatatag ctgtgaggga aatatataca
     4561 aatatattgg agcaaataaa ttgtacatac aaatatttat tactaatttc tattgagacg
     4621 aaatgaacca ctcggaacca tttgagcgaa ccgaatcgcg cggaactaac gacagtcgct
     4681 ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga cagcgaaaga
     4741 gcaactacga aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg atttgaaaag
     4801 tatgtatata aaaaatatat cccggtgttt tatgtagcga taaacgagtt tttgatgtaa
     4861 ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc caaagtctac ttgtggggat
     4921 gttcgaaggg gaaatacttg tattctatag gtcatatctt gtttttattg gcacaaatat
     4981 aattacatta gctttttgag ggggcaataa acagtaaaca cgatggtaat aatggtaaaa
     5041 aaaaaaaaca agcagttatt tcggatatat gtcggctact ccttgcgtcg ggcccgaagt
     5101 cttagagcca gatatgcgag cacccggaag ctcacgatga gaatggccag acccacgtag
     5161 tccagcggca gatcggcggc ggagaagtta agcgtctcca ggatgacctt gcccgaactg
     5221 gggcacgtgg tgttcgacga tgtgcagcta atttcgcccg gctccacgtc cgcccattgg
     5281 ttaatcagca gaccctcgtt ggcgtaacgg aaccatgaga ggtacgacaa ccatttgagg
     5341 tatactggca ccgagcccga gttcaagaag aagccgccaa agagcaggaa tggtatgata
     5401 accggcggac ccacagacag cgccatcgag gtcgaggagc tggcgcagga tattagatat
     5461 ccgaaggacg ttgacacatt ggccaccaga gtgaccagcg ccaggcagtt gaagaagtgc
     5521 agcactccgg cccgcagtcc gatcatcgga taggcaatcg ccgtgaagac cagtggcact
     5581 gtgagaaaaa gcggcaattc ggcaatcgtt ttgcccagaa agtatgtgtc acagcgataa
     5641 agtcgacttc gggcctccct cataaaaact ggcagctctg aggtgaacac ctaaatcgaa
     5701 tcgattcatt agaaagttag taaattattg aaatgcaaat gtattctaaa catgacttac
     5761 atttatcgtg gcaaagacgt tttgaaaggt catgttggtc aggaagagga agatggctcc
     5821 gttgatattc atcacaccca cttgcgtgag ttgttggccc aaaaagatga ggccaatcaa
     5881 gatggcaacc atctgcaaat taaaatgtta ctcgcatctc attaatattc gcgagttaaa
     5941 tgaaatttat ttatcttctg caaaactata aactatacat ctcattgaaa aaaactaaga
     6001 agggtgtgga atcaggcaat tctatctaaa atctagcgaa tttgtttcca agaattgtaa
     6061 gcgttatatc atttgtttcc actggaacca ctcaccgttg tctgaataag tcgcactttt
     6121 acgaggagtg gttccttgag caccgacagc caggatcgcc acaggaccgc ccggaactgc
     6181 atgaaccagg tggccttgta ggtgtaccca ttctccggct gctccagtgg cttctccaga
     6241 tttttggtgg ccaacaactg ctccatatcc cgggctactt tgctaatggc aaaattgtcg
     6301 catatcttgg cgatccgatc acgggactcg atctcccgtc cgggcacaac ggccaacacc
     6361 tgtacgtaaa agtccgccgg attgtagttg gtaggacact gggcacccac gctggatagg
     6421 agttgagatg ttatgtaata ctagataccc ttaataaaca catcgaactc actaggaaaa
     6481 gaagtcgacg gcttcgctgg gagtgcccaa gaaagctacc ctgccctcgg ccatcagaag
     6541 gatcttgtca aagagctcaa acagctcgga agacggctga tgaatggtca ggatgacggt
     6601 cttgcccttc tgcgacagct tcttcagcac ctggacgacg ctgtgggcgg taaaggagtc
     6661 cagtccggag gtgggctcat cgcagatcag aagcggcgga tcggttagag cctcggaggc
     6721 gaatgccaga cgcttccttt ctccgccgga cagacctttc accctgccgg gcacaccgat
     6781 gatcgtgtgc tgacatttgc tgagcgaaag ctcctggatc acctgatcca cgcgggccac
     6841 tcgctgccga taggtcagat gtcgtggcat ccgcaccatg gcttggaaaa tcaggtgttc
     6901 cctggccgtt agggagccga taaagaggtc atcctgctgg acataggcgc acctggcctg
     6961 catctccttg gcgtccacag gttggccatt gagcagtcgc atcccggatg gcgatacttg
     7021 gatgccctgc ggcgatcgaa aggcaagggc attcagcagg gtcgtctttc cggcaccgga
     7081 actgcccatc acggccaaaa gttcgcccgg ataggccacg ccgcaaactg agtttcaaat
     7141 tggtaattgg accctttatt aagatttcac acagatcagc cgactgcgaa tagaaactca
     7201 ccgttcttga gcaaatgttt cctgggcgcc ggtatgtgtc gctcgttgca gaatagtccg
     7261 cgtgtccggt tgaccagctg ccgccatccg gagcccggct gattgaccgc cccaaagatg
     7321 tccatattgt gccaggcata ggtgaggttc tcggctagtt ggccgctccc tgaaccggag
     7381 tcctccggcg gactgggtgg ccggagcgtg ccgtagtttt tggcctgccc gaagccctgg
     7441 ttaatgcagc tctgcgaagc cgctccgctg tcaccctgca atgatagggg atctcaaata
     7501 tcaactacta gcgttatgct catctaaccc cgaacaaaaa gtaccccgaa gtatcctacg
     7561 aagtaggttt atacttttat ttattttttg tgcatctagg atcagcttaa aatatctggt
     7621 tgttatattt tttgtaaaaa agaatatagt cgaaaatgaa tgcctttaga tgtcttgatc
     7681 atgatatgat ctcaaaaatt gtcttatata gcgagaacag ctaccagaat aatctgtttc
     7741 gtgtcactat ttgtttgtgc aattgcggtt tgggattttt gtgggtcgca gttctcacgc
     7801 cgcatacaat ttgatgttgc aatcgcagtt cctatagatc aagtgaactt aagatgtatg
     7861 cacatgtact actcacattg ttcagatgct cggcagatgg gtgtttgctg cctccgcgaa
     7921 ttaatagctc ctgatcctct tggcccattg ccgggatttt tcacactttc ccctgcttac
     7981 ccacccaaaa ccaatcacca ccccaatcac tcaaaaaaca aacaaaaata agaagcgaga
     8041 ggagttttgg cacagcactt tgtgtttaat tgatggcgta aaccgcttgg agcttcgtca
     8101 cgaaaccgct gacaaaatgc aactgaaggc ggacattgac gctacgtaac gctacaaacg
     8161 gtggcgaaag agatagcgga cgcagcggcg aaagagacgg cgatatttct gtggacagag
     8221 aaggaggcaa acagcgctga ctttgagtgg aatgtcattt tgagtgagag gtaatcgaaa
     8281 gaacctggta catcaaatac ccttggatcg aagtaaattt aaaactgatc agataagttc
     8341 aatgatatcc agtgcagtaa aaaaaaaaaa tgtttttttt atctactttc cgcaaaaatg
     8401 ggttttatta acttacatac atactaggcg cgcccatatg ttcggcttgt cgacatgccc
     8461 gccgtgaccg tcgagaaccc gctgacgctg ccccgcgtat ccgcacccgc cgacgccgtc
     8521 gcacgtcccg tgctcaccgt gaccaccgcg cccagcggtt tcgagggcga gggcttcccg
     8581 gtgcgccgcg cgttcgccgg gatcaactac cgccacctcg acccgttcat catgatggac
     8641 cagatgggtg aggtggagta cgcgcccggg gagcccaagg gcacgccctg gcacccgcac
     8701 cgcggcttcg agaccgtgac ctacatcgtc gacggtaaca tgtgagcaaa aggccagcaa
     8761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9661 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9841 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10141 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
