LOCUS       sequence-158331-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-HA-PuroR
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             97..150
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(785..804)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 tacccctacg acgtgcccga ctacgcctcc ggaggagagg gcagaggaag tctgctaaca
      121 tgcggtgacg tcgaggagaa tcctggccca atgactgaat acaagcctac tgtcaggttg
      181 gctacaagag acgacgttcc tagagccgtg agaactctgg ctgcagcctt cgccgactac
      241 cccgccacga gacacaccgt tgacccagat cggcatattg agagagtgac tgaactgcag
      301 gagctgtttc ttacaagagt tggcctcgac ataggcaagg tgtgggtggc ggacgacggc
      361 gccgccgtgg ccgtctggac cactcccgaa tcagttgagg ctggcgccgt attcgctgag
      421 atcggcccga gaatggctga gctcagcggg agtaggctcg cggcacagca gcaaatggag
      481 ggactgctgg caccacacag gcccaaagaa cccgcctggt tcctggcaac cgtcggtgta
      541 tctcccgatc atcaggggaa aggtctgggc tctgccgtag tgctccctgg cgtggaggca
      601 gctgagagag caggagtacc tgccttcttg gagacctccg ctccaaggaa tcttcccttc
      661 tatgaacggt tgggcttcac cgtgacagcc gacgtggaag tccccgaagg cccccgcact
      721 tggtgcatga cgaggaagcc tggagcgtga gggcccgctt cgagcagaca tgataagata
      781 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct tt
//
