LOCUS       sequence-158332-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-HA-PuroR
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(608..661)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(671..697)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(671..697)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    761..852
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(793..812)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    866..914
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgggccctca cgctccaggc ttcctcgtca tgcaccaagt gcgggggcct tcggggactt
       61 ccacgtcggc tgtcacggtg aagcccaacc gttcatagaa gggaagattc cttggagcgg
      121 aggtctccaa gaaggcaggt actcctgctc tctcagctgc ctccacgcca gggagcacta
      181 cggcagagcc cagacctttc ccctgatgat cgggagatac accgacggtt gccaggaacc
      241 aggcgggttc tttgggcctg tgtggtgcca gcagtccctc catttgctgc tgtgccgcga
      301 gcctactccc gctgagctca gccattctcg ggccgatctc agcgaatacg gcgccagcct
      361 caactgattc gggagtggtc cagacggcca cggcggcgcc gtcgtccgcc acccacacct
      421 tgcctatgtc gaggccaact cttgtaagaa acagctcctg cagttcagtc actctctcaa
      481 tatgccgatc tgggtcaacg gtgtgtctcg tggcggggta gtcggcgaag gctgcagcca
      541 gagttctcac ggctctagga acgtcgtctc ttgtagccaa cctgacagta ggcttgtatt
      601 cagtcattgg gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct
      661 ctcctccgga ggcgtagtcg ggcacgtcgt aggggtagga tccccctcca ctgccaccag
      721 accccccgct ttttgggtac tggcccgcta gctatcatta agagaaatgt tctggcacct
      781 gcacttgcac tggggacagc ctattttgct agtttgtttt gtttcgtttt gttttgatgg
      841 agagcgtatg ttagtactat cgattcacac aaaaaaccaa cacacagatg taatgaaaat
      901 aaagatattt tattgcggcc tgtccaatac ctcccgtacc ttaatattac ttacttatcc
      961 ttgagagacg tactagtaac c
//
