LOCUS       sequence-158335-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter: Encodes Histone H2B (125 aa) fused to smFLAG 
            (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis
            LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ
  TITLE     Real-time quantification of single RNA translation dynamics in 
            living cells.
  JOURNAL   Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899.
            Epub 2016 May 5.
  PUBMED    27313040
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaf0899"; journalName: "Science"; date: 
            "2016-06-17- 17"; volume: "352"; issue: "6292"; pages: "1425-9"
COMMENT     Sequence Label: pUB_smFLAG_H2B_MS2
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      69..78
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             78..101
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             132..155
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             660..683
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             690..713
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             726..749
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             756..779
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             972..995
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             999..1022
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
       61 tggcggccgc caccatggac tacaaggacg acgacgacaa aggtgactac aaagatgatg
      121 acgataaagg cgactataag gacgatgacg acaagggcgg aaactcactg atcaaggaaa
      181 acatgcggat gaaggtggtg atggagggct ccgtgaatgg tcaccagttc aagtgcaccg
      241 gagagggaga gggaaacccg tacatgggaa ctcagaccat gcgcattaag gtcatcgaag
      301 gaggtccgct gccgttcgct ttcgatatcc tggccacttc gttcggagga gggtcgcgca
      361 cgttcatcaa gtacccgaag ggaatcccgg acttctttaa gcagtcattc ccggaaggat
      421 tcacttggga acgggtgacc cggtatgaag atggaggtgt ggtgactgtc atgcaagata
      481 cttcgctgga ggatgggtgc ctcgtgtacc acgtccaagt ccgcggagtg aatttcccgt
      541 ccaacggacc agtgatgcag aaaaagacga agggttggga acctaatact gaaatgatgt
      601 accccgcaga cggagggctg aggggctaca cccacatggc gctgaaggtc gacggaggag
      661 attacaagga tgacgacgat aagcaacaag attacaaaga cgatgatgac aagggccagc
      721 agggcgacta caaggacgac gacgacaagc agcaggacta caaagatgac gatgataaag
      781 gaggaggaca tctgtcctgt tcgttcgtga ccacctacag atcaaagaaa accgtgggaa
      841 acatcaagat gccgggcatt catgccgtcg accaccgcct ggagcggctc gaagaatcag
      901 acaatgagat gttcgtcgtg caaagagaac atgccgtggc caagttcgcg ggactgggag
      961 gcggtggagg cgattacaaa gacgatgatg acaagggtga ctataaagac gacgatgaca
     1021 aaggggatta c
//
