LOCUS       sequence-158336-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter: Encodes Histone H2B (125 aa) fused to smFLAG 
            (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis
            LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ
  TITLE     Real-time quantification of single RNA translation dynamics in 
            living cells.
  JOURNAL   Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899.
            Epub 2016 May 5.
  PUBMED    27313040
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaf0899"; journalName: "Science"; date: 
            "2016-06-17- 17"; volume: "352"; issue: "6292"; pages: "1425-9"
COMMENT     Sequence Label: pUB_smFLAG_H2B_MS2
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aattttgtaa tccagaggtt gattaggatc tatcgatatg agatctgagg tgtttgatgt
       61 acggtgcggt gatcctgcac cgtttgcctg gttgtaagtt tgtgggttgt cggcggatgc
      121 cgtgttcgtg catccgtttg tcgtgttttt gtgaggtttt ccgccgcggg aaggctgttg
      181 ctcttcccgt cggatgtttt tcgtaatagt ttgcgctgga cgaaagcgtg atcgtctttc
      241 gtatttgtaa accagttgat ttttccgtgt gtaccgttcg ctgttgctga acggtttggt
      301 ttttgatagt agtgagagat gtgggctggt acgctgatgc tgtaccagta attgtttttg
      361 atttggtatg ttggaatggg caagcggtga tcctgcttgc cagttttgtg ggaagaggtt
      421 tgaggtagga gtgggttcgc tggtgctgaa cccatttggt agtttctgtg gttgagactt
      481 gtggagcatg ccgtgatcgt gcatgcttga atgtcctgag tggaagtgat gtagctgcgg
      541 gatccgtgat cgtgatcccg aaaggtaagt aatcgtattg gacgtggaac gacgagccct
      601 ggtggtgctc gtccttgtcc gtgttttgaa attcgtgaga gcatgggtgg cgtgatcgtc
      661 cacccaagaa ataggcacaa tgtatgctcg agtgtttcga agcgctgatg ctgcttcgag
      721 aagagtagtt tttgtcttgt tggtgagagt ccatggcctg atggtccatg
//
