LOCUS       sequence-158337-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-myc-PuroR
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..101
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             111..164
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(799..818)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 atttctctta atgatagcta gcgggccagt acccaaaaag cggggggtct ggtggcagtg
       61 gagggggatc cgagcagaag ctgatcagcg aggaggacct gtccggagga gagggcagag
      121 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaatgact gaatacaagc
      181 ctactgtcag gttggctaca agagacgacg ttcctagagc cgtgagaact ctggctgcag
      241 ccttcgccga ctaccccgcc acgagacaca ccgttgaccc agatcggcat attgagagag
      301 tgactgaact gcaggagctg tttcttacaa gagttggcct cgacataggc aaggtgtggg
      361 tggcggacga cggcgccgcc gtggccgtct ggaccactcc cgaatcagtt gaggctggcg
      421 ccgtattcgc tgagatcggc ccgagaatgg ctgagctcag cgggagtagg ctcgcggcac
      481 agcagcaaat ggagggactg ctggcaccac acaggcccaa agaacccgcc tggttcctgg
      541 caaccgtcgg tgtatctccc gatcatcagg ggaaaggtct gggctctgcc gtagtgctcc
      601 ctggcgtgga ggcagctgag agagcaggag tacctgcctt cttggagacc tccgctccaa
      661 ggaatcttcc cttctatgaa cggttgggct tcaccgtgac agccgacgtg gaagtccccg
      721 aaggcccccg cacttggtgc atgacgagga agcctggagc gtgagggccc gcttcgagca
      781 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca g
//
