LOCUS       sequence-158339-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETconNK with TEM1(S70A,D179G) beta-lactamase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA
  TITLE     Trade-offs between enzyme fitness and solubility illuminated by deep
            mutational scanning.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114.
  PUBMED    28196882
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114."
COMMENT     Sequence Label: pETconNK-TEM1(S70A,D179G)
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(294..323)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cattcatctt tttttagcta ctaataggat aaattatagg aatttataac aaattgaaat
       61 atggcaggca gcaaaattaa aattgtctta gttttttagt gtatagaagt gaatagctat
      121 ataaagtatg tgtaaagttg gtaacggaac gaaaaataga aaaggatatt acatgggaaa
      181 acatgttgtt tacggagaaa tgaaaagtat attgtatttt gtacgagcta aaagtacagt
      241 gggaacaaag tcgattttgt tacatctaca ctgttgttat cagatctcta ttacaagtcc
      301 tcttcagaaa taagcttttg ttcggatccg cccccctcga gccaatgctt aatcagtgag
      361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
      421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
      481 gacccacgct cacctgctcc agatttatca gcaataaacc agccagccgg aagggccgag
      541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
      601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
      661 atcgtggtgc cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
      721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
      781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
      841 aattctctta ctgtcatgcc atccgtaaga tgctt
//
