LOCUS       sequence-158340-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-NanoLuc-PuroR
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..578
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     CDS             588..641
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tctcttaatg atagctagcg ggccagtacc caaaaagcgg ggggtctggt ggcagtggag
       61 ggggatccgt cttcacactc gaagatttcg ttggggactg gcgacagaca gccggctaca
      121 acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat ctcggggtgt
      181 ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag atcgacatcc
      241 atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc gaaaaaattt
      301 ttaaggtggt gtaccctgtg gatgatcatc actttaaggt gatcctgcac tatggcacac
      361 tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg tatgaaggca
      421 tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac ggcaacaaaa
      481 ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta accatcaacg
      541 gagtgaccgg ctggcggctg tgcgaacgca ttctggcgtc cggaggagag ggcagaggaa
      601 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aatgactgaa tacaagccta
      661 ctgtcaggtt ggctacaaga gacgacgttc ctagagccgt gagaactctg gctgcagcct
      721 tcgccgacta ccccgccacg agacacaccg ttgacccaga tcggcatatt gagagagtga
      781 ctgaactgca ggagctgttt cttacaagag ttggcctcga cataggcaag gtgtgggtgg
      841 cggacgacgg cgccgccgtg gccgtctgga ccactcccga atcagttgag gctggcgccg
      901 tattc
//
