LOCUS       sequence-158343-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-nTEV-PuroR
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             424..477
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 ggcgagagcc tgttcaaggg acccagagac tacaacccca tcagcagcac catctgccac
      121 ctgaccaacg agagcgacgg ccacaccacc agcctgtacg gcatcggctt cggccccttc
      181 atcatcacca acaagcacct gttcagaaga aacaacggca ccctgctggt gcagagcctg
      241 cacggcgtgt tcaaggtgaa gaacaccacc accctgcagc agcacctgat cgacggcaga
      301 gacatgatca tcatcagaat gcccaaggac ttccccccct tcccccagaa gctgaagttc
      361 agagagcccc agagagagga gagaatctgc ctggtgacca ccaacttcca gacctccgga
      421 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg
      481 actgaataca agcctactgt caggttggct acaagagacg acgttcctag agccgtgaga
      541 actctggctg cagccttcgc cgactacccc gccacgagac acaccgttga cccagatcgg
      601 catattgaga gagtgactga actgcaggag ctgtttctta caagagttgg cctcgacata
      661 ggcaaggtgt gggtggcgga cgacggcgcc gccgtggccg tctggaccac tcccgaatca
      721 gttgaggctg gcgccgtatt cgctgagatc ggcccgagaa tggctgagct cagcgggagt
      781 aggctcgcgg cacagca
//
