LOCUS       sequence-158344-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-nTEV-PuroR
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(598..651)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cgctccaggc ttcctcgtca tgcaccaagt gcgggggcct tcggggactt ccacgtcggc
       61 tgtcacggtg aagcccaacc gttcatagaa gggaagattc cttggagcgg aggtctccaa
      121 gaaggcaggt actcctgctc tctcagctgc ctccacgcca gggagcacta cggcagagcc
      181 cagacctttc ccctgatgat cgggagatac accgacggtt gccaggaacc aggcgggttc
      241 tttgggcctg tgtggtgcca gcagtccctc catttgctgc tgtgccgcga gcctactccc
      301 gctgagctca gccattctcg ggccgatctc agcgaatacg gcgccagcct caactgattc
      361 gggagtggtc cagacggcca cggcggcgcc gtcgtccgcc acccacacct tgcctatgtc
      421 gaggccaact cttgtaagaa acagctcctg cagttcagtc actctctcaa tatgccgatc
      481 tgggtcaacg gtgtgtctcg tggcggggta gtcggcgaag gctgcagcca gagttctcac
      541 ggctctagga acgtcgtctc ttgtagccaa cctgacagta ggcttgtatt cagtcattgg
      601 gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct ctcctccgga
      661 ggtctggaag ttggtggtca ccaggcagat tctctcctct ctctggggct ctctgaactt
      721 cagcttctgg gggaaggggg ggaagtcctt gggcattctg atgatgatca tgtctctgcc
      781 gtcgatcagg tgctgctgca gggtggtggt gttcttcacc ttgaacacgc cgtgcaggct
      841 ctgcaccagc agggtgccgt tgtttcttct gaacaggtgc ttgttggtga tgatgaa
//
