LOCUS       sequence-158346-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-ProteoTuner DD-PuroR
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..381
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLGDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQGAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     ELE"
     CDS             391..444
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
      121 acctgcgtgg tgcactacac cgggatgctt ggagatggaa agaaagttga ctcctcccgg
      181 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      241 gaaggggttg cccagatgag tgtgggtcag ggagccaaac tgactatatc tccagattat
      301 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      361 gtggagcttc tagaactgga atccggagga gagggcagag gaagtctgct aacatgcggt
      421 gacgtcgagg agaatcctgg cccaatgact gaatacaagc ctactgtcag gttggctaca
      481 agagacgacg ttcctagagc cgtgagaact ctggctgcag ccttcgccga ctaccccgcc
      541 acgagacaca ccgttgaccc agatcggcat attgagagag tgactgaact gcaggagctg
      601 tttcttacaa gagttggcct cgacataggc aaggtgtggg tggcggacga cggcgccgcc
      661 gtggccgtct ggaccactcc cgaatcagtt gaggctggcg ccgtattcgc tgagatcggc
      721 ccgagaatgg ctgagctcag cgggagtagg ctcgcggcac agcagcaaat ggagggactg
      781 ctggcaccac acaggcccaa agaacccgcc tggttcctgg caaccgtcgg tgtatctccc
      841 gatcatcagg ggaaaggtct gggctctgcc gta
//
