LOCUS       sequence-158347-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETconNK with TEM1(S70A,D179G) beta-lactamase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA
  TITLE     Trade-offs between enzyme fitness and solubility illuminated by deep
            mutational scanning.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114.
  PUBMED    28196882
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114."
COMMENT     Sequence Label: pETconNK-TEM1(S70A,D179G)
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     75..98
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             430..441
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             442..468
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(687..706)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actaatactt tcaacatttt cggtttgtat tacttcttat tcaaatgtaa taaaagtatc
       61 aacaaaaaat tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ccccggatcg
      121 aattccctac ttcatacatt ttcaattaag atgcagttac ttcgctgttt ttcaatattt
      181 tctgttattg cttcagtttt agcacaggaa ctgacaacta tatgcgagca aatcccctca
      241 ccaactttag aatcgacgcc gtactctttg tcaacgacta ctattttggc caacgggaag
      301 gcaatgcaag gagtttttga atattacaaa tcagtaacgt ttgtcagtaa ttgcggttct
      361 cacccctcaa caactagcaa aggcagcccc ataaacacac agtatgtttt taaggacaat
      421 agctcgacga ttgaaggtag atacccatac gacgttccag actacgctct gcaggctagt
      481 ggtggaggag gctctggtgg aggcggtagc ggaggcggag ggtcggctag ccatatgcac
      541 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      601 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      661 ccaatgatgg ccacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
      721 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      781 ccagtcacag aaaagcatct tacggatggc atgacagtaa ga
//
