LOCUS       sequence-158349-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter: Encodes KDM5B (1544 aa) fused to smHA 
            (Spaghetti Monster HA: 10 copies of an epitope tag HA)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis
            LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ
  TITLE     Real-time quantification of single RNA translation dynamics in 
            living cells.
  JOURNAL   Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899.
            Epub 2016 May 5.
  PUBMED    27313040
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaf0899"; journalName: "Science"; date: 
            "2016-06-17- 17"; volume: "352"; issue: "6292"; pages: "1425-9"
COMMENT     Sequence Label: pUB_smHA_KDM5B_MS2
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             108..134
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             138..164
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             693..719
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             726..752
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             765..791
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             798..824
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
       61 tggcggccgc caccatgtac ccttatgatg tgcccgatta tgctggctac ccttatgatg
      121 ttcctgatta cgccggatat ccgtatgacg tgccagacta cgcgggaggt gtgagcaagg
      181 gagaggagtt gtttaccggc gtggtcccca tactggtgga gttggatggc gacgttaatg
      241 gacataaatt ctcggtccgc ggcgagggag agggagacgc caccaatggc aagctgacgc
      301 ttaagttcat ttgcacgact ggaaaattgc ctgtcccctg gccgaccctg gtcaccacac
      361 tgggcggagg agtgcagtgc ttctcccgtt acccagacca catgaagcag cacgatttct
      421 tcaagagcgc aatgccggag ggatacgtgc aagaacggac tatttccttc aaggatgacg
      481 gaacctataa gacccgtgcg gaggtcaaat tcgagggtga caccctggtg aaccgaattg
      541 aactcaaagg aatcgatttc aaggaggatg gaaatattct gggtcacaag ctggaataca
      601 acttcaacag ccataatgtg tacattacgg ctgataagca gaagaacggc attaaggcca
      661 atttcaagat ccgccacaac gttgagggtg gatatcccta cgacgtgccc gattatgcgg
      721 gcggatatcc gtatgatgtt ccagattatg ctggtggtgg aggctatccc tatgatgtcc
      781 ccgactacgc cggaggttac ccatacgacg tgcccgacta cgctggaggc gatggcagcg
      841 tgcagctggc agatcattat caacagaata cccccatagg tgatggcccc gttctgcttc
      901 cagataatca ctacctttcc acccagagcg tgctttcgaa agacccgaac gaaaagcgtg
      961 atcacatg
//
