LOCUS       sequence-158350-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-SBP-PuroR
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..174
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             184..237
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 atggacgaaa agactactgg ctggcgcgga ggacacgtcg tcgagggact tgctggggag
      121 ctggaacagc ttcgagctag gctcgagcac cacccccagg ggcaaagaga gccctccgga
      181 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg
      241 actgaataca agcctactgt caggttggct acaagagacg acgttcctag agccgtgaga
      301 actctggctg cagccttcgc cgactacccc gccacgagac acaccgttga cccagatcgg
      361 catattgaga gagtgactga actgcaggag ctgtttctta caagagttgg cctcgacata
      421 ggcaaggtgt gggtggcgga cgacggcgcc gccgtggccg tctggaccac tcccgaatca
      481 gttgaggctg gcgccgtatt cgctgagatc ggcccgagaa tggctgagct cagcgggagt
      541 aggctcgcgg cacagcagca aatggaggga ctgctggcac cacacaggcc caaagaaccc
      601 gcctggttcc tggcaaccgt cggtgtatct cccgatcatc aggggaaagg tctgggctct
      661 gccgtagtgc tccctggcgt ggaggcagct gagagagcag gagtacctgc cttcttggag
      721 acctccgctc caaggaatct tcccttctat gaacggttgg gcttcaccgt gacagccgac
      781 gtggaagtcc ccgaaggccc ccgcacttgg tgcatgacga ggaagcctgg agcgt
//
