LOCUS       sequence-158360-        3045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for inducible expression of LacZ with 
            N-terminal 3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.10"; journalName: "Dev Cell"; date: 
            "2017-11-20- 20"; volume: "43"; issue: "4"; pages: "418-435.e13"
COMMENT     Sequence Label: pEN_TT 3xFLAG LacZ
FEATURES             Location/Qualifiers
     source          1..3045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..3045
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(3..25)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
       61 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      121 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
      181 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca
      241 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg
      301 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
      361 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg
      421 gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg
      481 tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg
      541 ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt
      601 ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc
      661 actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc
      721 gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc
      781 agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc
      841 gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc
      901 tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc
      961 gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg
     1021 ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat
     1081 gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc
     1141 tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat
     1201 gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat
     1261 gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt
     1321 aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac
     1381 gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc
     1441 ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa
     1501 gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga
     1561 gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt
     1621 ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg
     1681 gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac
     1741 ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt
     1801 gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag
     1861 ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat
     1921 aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg
     1981 cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg
     2041 gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg
     2101 tcagaagccg gccacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg
     2161 acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc
     2221 atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg
     2281 tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg
     2341 ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa
     2401 cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat
     2461 acacttgctg acgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc
     2521 ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt
     2581 gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg
     2641 gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac
     2701 cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg
     2761 tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca
     2821 caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg
     2881 gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt
     2941 ttccatatgg ggattggtgg agacgactcc tggagcccgt cagtatcggc ggaattacag
     3001 ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaa
//
