LOCUS       sequence-158363-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A ZipGFP-based Caspase-3 protease reporter T2A mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   To TL, Schepis A, Ruiz-Gonzalez R, Zhang Q, Yu D, Dong Z, Coughlin 
            SR, Shu X
  TITLE     Rational Design of a GFP-Based Fluorogenic Caspase Reporter for 
            Imaging Apoptosis In Vivo.
  JOURNAL   Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 
            10.1016/j.chembiol.2016.06.007.
  PUBMED    27447051
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.06"; journalName: "Cell Chem Biol"; date: 
            "2016-07-21- 21"; volume: "23"; issue: "7"; pages: "875-82"
COMMENT     Sequence Label: ZipGFP-Casp3
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             135..182
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             306..359
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      366..375
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttaacttagc ttgccccatg ggcggcagca aggtgtctgc acttaaagaa aaggtttccg
       61 cactcaagga gaaagtttca gctttgaaag agaaggtcag tgcccttaag gaaaaagtga
      121 gcgcgctgaa agaacgggac cacatggtgc tgcacgagta cgtgaacgcc gccggcatca
      181 caggtgatga agttgatggt gaagtgagcg cgctggaaaa agaggtctcc gctcttgaga
      241 aggaagtttc tgccttggag aaagaggtga gcgccctgga gaaggaagtc tctgcgcttg
      301 aaaaagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggcccaa
      361 agcttgccac catggaagtg agcgcgctgg aaaaagaggt ctccgctctt gagaaggaag
      421 tttctgcctt ggagaaagag gtgagcgccc tggagaagga agtctctgcg cttgaaaaag
      481 gatcctccaa aggagaagaa ctgtttaccg gtgttgtgcc aattttggtt gaactcgatg
      541 gtgatgtcaa cggacataag ttctcagtga gaggcgaagg agaaggtgac gccaccattg
      601 gaaaattgac tcttaaattc atctgtacta ctggtaaact tcctgtacca tggccgactc
      661 tcgtaacaac gcttacgtac ggagttcagt gcttttcgag atacccagac catatgaaaa
      721 gacatgactt ttttaagtcg gctatgcctg aaggttacgt gcaagaaaga acaatttcgt
      781 tcaaagatga tggaaaatat aaaactagag cagttgttaa atttgaagga gatactttgg
      841 ttaaccgcat tgaactgaaa ggaacagatt ttaaagaaga tggtaatatt cttggacaca
      901 aactcgaata caattttaat agtcataacg tatacatcac tgctgataag caaaagaacg
      961 gaattaaagc gaatttcaca gtacgccata atgtagaaga tggcagtgtt caacttgccg
     1021 accattacca acaaa
//
