LOCUS       sequence-158364-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A ZipGFP-based Caspase-3 protease reporter T2A mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   To TL, Schepis A, Ruiz-Gonzalez R, Zhang Q, Yu D, Dong Z, Coughlin 
            SR, Shu X
  TITLE     Rational Design of a GFP-Based Fluorogenic Caspase Reporter for 
            Imaging Apoptosis In Vivo.
  JOURNAL   Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 
            10.1016/j.chembiol.2016.06.007.
  PUBMED    27447051
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.06"; journalName: "Cell Chem Biol"; date: 
            "2016-07-21- 21"; volume: "23"; issue: "7"; pages: "875-82"
COMMENT     Sequence Label: ZipGFP-Casp3
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..722)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(290..309)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     559..577
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(729..782)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggccctctag attacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
       61 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg
      121 ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc
      181 tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg
      241 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc
      301 attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg
      361 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
      421 acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg
      481 gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
      541 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
      601 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
      661 accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc
      721 atcaattctg ggccaggatt ctcctcgacg tcaccgcatg ttagcagact tcctctgccc
      781 tctctagagc ggccgccttc tttcagcgcg ctcacttttt ccttaagggc actgaccttc
      841 tctttcaaag ctgaaacttt ctccttgagt gcggaaacct tttctttaag tgcagacacc
      901 ttgctgccgc cgaattcacc atcaacttca tcaccttttt catttggatc tttgctcagg
      961 actgtttgtg ttgagaggta gtgattatca ggaagaagta
//
