LOCUS       sequence-158365-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zipped GFP1-10 with TEV cleavage sequence T2A mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   To TL, Schepis A, Ruiz-Gonzalez R, Zhang Q, Yu D, Dong Z, Coughlin 
            SR, Shu X
  TITLE     Rational Design of a GFP-Based Fluorogenic Caspase Reporter for 
            Imaging Apoptosis In Vivo.
  JOURNAL   Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 
            10.1016/j.chembiol.2016.06.007.
  PUBMED    27447051
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.06"; journalName: "Cell Chem Biol"; date: 
            "2016-07-21- 21"; volume: "23"; issue: "7"; pages: "875-82"
COMMENT     Sequence Label: ZipGFP1-10_TEV
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acttaagctt gccaccatgg aagtgagcgc gctggaaaaa gaggtctccg ctcttgagaa
       61 ggaagtttct gccttggaga aagaggtgag cgccctggag aaggaagtct ctgcgcttga
      121 aaaaggatcc tccaaaggag aagaactgtt taccggtgtt gtgccaattt tggttgaact
      181 cgatggtgat gtcaacggac ataagttctc agtgagaggc gaaggagaag gtgacgccac
      241 cattggaaaa ttgactctta aattcatctg tactactggt aaacttcctg taccatggcc
      301 gactctcgta acaacgctta cgtacggagt tcagtgcttt tcgagatacc cagaccatat
      361 gaaaagacat gactttttta agtcggctat gcctgaaggt tacgtgcaag aaagaacaat
      421 ttcgttcaaa gatgatggaa aatataaaac tagagcagtt gttaaatttg aaggagatac
      481 tttggttaac cgcattgaac tgaaaggaac agattttaaa gaagatggta atattcttgg
      541 acacaaactc gaatacaatt ttaatagtca taacgtatac atcactgctg ataagcaaaa
      601 gaacggaatt aaagcgaatt tcacagtacg ccataatgta gaagatggca gtgttcaact
      661 tgccgaccat taccaacaaa acacccctat tggagacggt ccggtacttc ttcctgataa
      721 tcactacctc tcaacacaaa cagtcctgag caaagatcc
//
