LOCUS       sequence-158366-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zipped GFP1-10 with TEV cleavage sequence T2A mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   To TL, Schepis A, Ruiz-Gonzalez R, Zhang Q, Yu D, Dong Z, Coughlin 
            SR, Shu X
  TITLE     Rational Design of a GFP-Based Fluorogenic Caspase Reporter for 
            Imaging Apoptosis In Vivo.
  JOURNAL   Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 
            10.1016/j.chembiol.2016.06.007.
  PUBMED    27447051
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.06"; journalName: "Cell Chem Biol"; date: 
            "2016-07-21- 21"; volume: "23"; issue: "7"; pages: "875-82"
COMMENT     Sequence Label: ZipGFP1-10_TEV
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..717)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(285..304)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     554..572
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(724..777)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(913..933)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tctagattac ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc
       61 gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac
      121 gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc
      181 gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc
      241 gtcctcgggg tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac
      301 ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc
      361 ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg
      421 ctcccacttg aagccctcgg ggaaggacag cttcaagtag tcggggatgt cggcggggtg
      481 cttcacgtag gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg
      541 cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc
      601 ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt
      661 gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatcaa
      721 ttctgggcca ggattctcct cgacgtcacc gcatgttagc agacttcctc tgccctctct
      781 agagcggccg ccttctttca gcgcgctcac tttttcctta agggcactga ccttctcttt
      841 caaagctgaa actttctcct tgagtgcgga aaccttttct ttaagtgcag acaccttgct
      901 gccgccgaat tcactctgga agtagagatt ttctttttca tttggatctt tgctcaggac
      961 tgtttgtgtt gagaggtagt gattatcagg aaga
//
