LOCUS       sequence-158367-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zipped GFP11 with TEV cleavage sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   To TL, Schepis A, Ruiz-Gonzalez R, Zhang Q, Yu D, Dong Z, Coughlin 
            SR, Shu X
  TITLE     Rational Design of a GFP-Based Fluorogenic Caspase Reporter for 
            Imaging Apoptosis In Vivo.
  JOURNAL   Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 
            10.1016/j.chembiol.2016.06.007.
  PUBMED    27447051
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.06"; journalName: "Cell Chem Biol"; date: 
            "2016-07-21- 21"; volume: "23"; issue: "7"; pages: "875-82"
COMMENT     Sequence Label: ZipGFP11_TEV
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             148..195
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(371..388)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    377..601
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(734..753)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cttaagcttg ccaccatggg cggcagcaag gtgtctgcac ttaaagaaaa ggtttccgca
       61 ctcaaggaga aagtttcagc tttgaaagag aaggtcagtg cccttaagga aaaagtgagc
      121 gcgctgaaag aaggaggcag cggatcccgg gaccacatgg tgctgcacga gtacgtgaac
      181 gccgccggca tcacagaaaa cctgtacttt cagtccctcg agggaggcag cgaagtgagc
      241 gcgctggaaa aagaggtctc cgctcttgag aaggaagttt ctgccttgga gaaagaggtg
      301 agcgccctgg agaaggaagt ctctgcgctt gaaaaataat ctagagggcc cgtttaaacc
      361 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      421 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      481 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      541 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      601 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
      661 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      721 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      781 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      841 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      901 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      961 actg
//
