LOCUS       sequence-158368-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zipped GFP11 with TEV cleavage sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   To TL, Schepis A, Ruiz-Gonzalez R, Zhang Q, Yu D, Dong Z, Coughlin 
            SR, Shu X
  TITLE     Rational Design of a GFP-Based Fluorogenic Caspase Reporter for 
            Imaging Apoptosis In Vivo.
  JOURNAL   Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 
            10.1016/j.chembiol.2016.06.007.
  PUBMED    27447051
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.06"; journalName: "Cell Chem Biol"; date: 
            "2016-07-21- 21"; volume: "23"; issue: "7"; pages: "875-82"
COMMENT     Sequence Label: ZipGFP11_TEV
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(130..150)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(151..198)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     regulatory      327..336
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(371..390)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(372..390)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(435..638)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(464..484)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        639..942
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tctagattat ttttcaagcg cagagacttc cttctccagg gcgctcacct ctttctccaa
       61 ggcagaaact tccttctcaa gagcggagac ctctttttcc agcgcgctca cttcgctgcc
      121 tccctcgagg gactgaaagt acaggttttc tgtgatgccg gcggcgttca cgtactcgtg
      181 cagcaccatg tggtcccggg atccgctgcc tccttctttc agcgcgctca ctttttcctt
      241 aagggcactg accttctctt tcaaagctga aactttctcc ttgagtgcgg aaaccttttc
      301 tttaagtgca gacaccttgc tgccgcccat ggtggcaagc ttaagtttaa acgctagcca
      361 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
      421 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      481 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
      541 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
      601 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
      661 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
      721 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
      781 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
      841 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
      901 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc cata
//
