LOCUS       sequence-158369-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETconNK with levoglucosan kinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA
  TITLE     Trade-offs between enzyme fitness and solubility illuminated by deep
            mutational scanning.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114.
  PUBMED    28196882
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114."
COMMENT     Sequence Label: pETconNK-LGK
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(294..323)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cattcatctt tttttagcta ctaataggat aaattatagg aatttataac aaattgaaat
       61 atggcaggca gcaaaattaa aattgtctta gttttttagt gtatagaagt gaatagctat
      121 ataaagtatg tgtaaagttg gtaacggaac gaaaaataga aaaggatatt acatgggaaa
      181 acatgttgtt tacggagaaa tgaaaagtat attgtatttt gtacgagcta aaagtacagt
      241 gggaacaaag tcgattttgt tacatctaca ctgttgttat cagatctcta ttacaagtcc
      301 tcttcagaaa taagcttttg ttcggatccg cccccctcga gcgcccagtt gttggtgata
      361 actttgcctt ttttcttcac gatcatttca gaaacccacg gcagttgctg agcatcgcca
      421 ccaaatgcca tgcctttttt catcacagag cggtaattca gacccgggct aactttgccc
      481 agcacatagt gctggcgcgt ttcgacacgg gtcggaaccg ggatgctacg accaaccagg
      541 gcttccatgc cctgccaggc gaacgtaatc gcttcttttg cacctgccgg gacacctgct
      601 tcatccagca tcataatttt ggtgttcggg tacgattgct ggataaattc cacaatattc
      661 gggttatagg cgccaccgcc gcacatgaag atttcgtcaa tttcttgtga cggcgcgtag
      721 cgacgatagt gatcaacgat agcctgtgcc gtaatacggg tcgtggtggc cacgatgtca
      781 tccgggctca gacctttggc ttccgcgcga cgaatcaggt catgtgccag g
//
