LOCUS       sequence-158371-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLIB - Library vector (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pLIB
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(12..45)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        93..184
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     109..126
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    443..577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(480..499)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     534..553
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gccgcggtac cataacttcg tatagcatac attatacgaa gttatctggt tttactagta
       61 tcgattcgcg acctactccg gaatattaat agatcatgga gataattaaa atgataacca
      121 tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat
      181 aaatattccg gattattcat accgtcccac catcgggcgc ggatcccggt ccgaagcgcg
      241 cggaattcaa aggcctacgt cgacgagctc actagtcgcg gccgctttcg aatctagagc
      301 ctgcagtctc gacaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt
      361 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      421 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      481 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      541 gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctagaag
      601 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      661 aactatcata acccctagga gatccgaacc agataagtga aatctagttc caaactattt
      721 tgtcattttt aattttcg
//
