LOCUS       sequence-158372-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLIB - Library vector (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pLIB
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  247..412
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    522..656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(546..565)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     600..619
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
       61 cgtcccattc gccattcagg ctgcaaataa gcgttgatat tcagtcaatt acaaacatta
      121 ataacgaaga gatgacagaa aaattttcat tctgtgacag agaaaaagta gccgaagatg
      181 acggtttgtc acatggagtt ggcaggatgt ttgattaaaa acataacagg aagaaaaatg
      241 ccccgctgtg ggcggacaaa atagttggga actgggaggg gtggaaatgg agtttttaag
      301 gattatttag ggaagagtga caaaatagat gggaactggg tgtagcgtcg taagctaata
      361 cgaaaattaa aaatgacaaa atagtttgga actagatttc acttatctgg ttcggatctc
      421 ctaggggtta tgatagttat tgctcagcgg tggcagcagc caactcagct tcctttcggg
      481 ctttgttagc agccggatct tctaggctca agcagtgatc agatccagac atgataagat
      541 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      601 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      661 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
      721 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagta cttctcgaca
      781 agcttgtcga gactgcaggc tctagattcg aaagcggccg cgactagtga gctcgtcgac
      841 gtaggccttt gaattccgcg cgcttcggac cgggatccgc gcccgatggt gggacggtat
      901 gaataatccg gaatatttat aggttttttt attacaaaac tgttacgaaa acagtaaaat
      961 acttatttat ttgcgaga
//
