LOCUS       sequence-158374-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP fusion to levoglucosan kinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Wrenbeck EE, Bedewitz MA, Klesmith JR, Noshin S, Barry CS, Whitehead
            TA
  TITLE     An Automated Data-Driven Pipeline for Improving Heterologous Enzyme 
            Expression.
  JOURNAL   ACS Synth Biol. 2019 Mar 15;8(3):474-481. doi: 
            10.1021/acssynbio.8b00486. Epub 2019 Feb 8.
  PUBMED    30721031
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00486"; journalName: "ACS Synth Biol"; date: 
            "2019-03-15- 15"; volume: "8"; issue: "3"; pages: "474-481"
COMMENT     Sequence Label: pET29NK-LGK/mGFPmut3
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gggtgataat gttctggact ttacggttct gggcctgaat agcggtacga gtatggatgg
       61 tattgactgc gcactgtgtc atttctatca gaaaaccccg gatgctccga tggaatttga
      121 actgctggaa tacggcgaag ttccgctggc gcagccgatt aaacaacgtg tcatgcgcat
      181 gatcctggaa gataccacga gcccgtctga actgtcagaa gtcaacgtga ttctgggtga
      241 acattttgcg gatgccgtcc gtcagttcgc ggccgaacgc aatgtggatc tgtcaaccat
      301 tgacgcaatc gcttcgcacg gccagacgat ttggctgctg agtatgccgg aagaaggtca
      361 agtgaaatcc gccctgacca tggcagaagg cgctatcctg gcgagtcgta cgggtattac
      421 ctccatcacg gatttccgta tttccgacca ggcagctggt cgtcaaggtg caccgctgat
      481 cgcatttttc gatgctctgc tgctgcatca cccgaccaaa ctgcgcgcgt gccagaacat
      541 tggcggtatc gccaatgtgt gttttattcc gccggatgtt gacggccgtc gcaccgatga
      601 atattacgat tttgacacgg gtccgggcaa cgtgttcatc gacgcagtgg ttcgtcattt
      661 taccaatggt gaacaggaat atgataaaga cggtgctatg ggcaaacgcg gtaaagtcga
      721 tca
//
