LOCUS       sequence-158378-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG1b - Step1 vector b (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG1b
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    361..409
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     434..453
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    499..532
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca aaatagtttg
       61 gaactagatt tcacttatct ggttcggatc tcctgtttaa acgtgcaata gtatccagtt
      121 tttaattaac ccgtagtggc tatggcaggg cttgccgccc cgacgttggc tgcgagccct
      181 gggccttcac ccgaacttgg gggttggggt ggggaaaagg aagaaacgcg ggcgtattgg
      241 tcccaatggg gtctcggtgg ggtatcgacg taaccccgat tgagatatag atttaaatct
      301 ttagaccata gagcgttcac ttgacagagt gccagccctg ggaccgaacc ccgcgtttat
      361 gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
      421 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcgataaaaa ccccgattga
      481 gatatagttt aaacccagat aacttcgtat aatgtatgct atacgaagtt atggtaccgc
      541 ggccgcgtag aggatctgtt gatcagcagt tcaacctgtt gatagtacaa acaaagctgt
      601 agccaaccac tagaactata gctagagtcc tgggcgaaca aacgatgctc gccttccaga
      661 aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc
      721 cgaggtctac cgatctcctg aagcaagggc agatccgtgc acagcacctt gccgtagaag
      781 aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc
      841 agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg
      901 tggaaacgga tgaaggca
//
