LOCUS       sequence-158385-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG1d - Step1 vector d (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG1d
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    362..410
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     435..454
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    500..533
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(929..934,935..951,952..957)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gatgggaact gggtgtagcg tcgtaagcta atacgaaaat taaaaatgac aaaatagttt
       61 ggaactagat ttcacttatc tggttcggat ctcctgttta aactaagcta tgtgaaccgt
      121 ttttaattaa cccgtagtgg ctatggcagg gcttgccgcc ccgacgttgg ctgcgagccc
      181 tgggccttca cccgaacttg ggggttgggg tggggaaaag gaagaaacgc gggcgtattg
      241 gtcccaatgg ggtctcggtg gggtatcgac gtaaccccga ttgagatata gatttaaatc
      301 tttagaccat agagcgttca cttgacagag tgccagccct gggaccgaac cccgcgttta
      361 tgaacaaacg acccaacacc cgtgcgtttt attctgtctt tttattgccg tcatagcgcg
      421 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctcgataaaa acatcaggca
      481 tcattaggtt taaacccaga taacttcgta taatgtatgc tatacgaagt tatggtaccg
      541 cggccgcgta gaggatctgt tgatcagcag ttcaacctgt tgatagtaca aacaaagctg
      601 tagccaacca ctagaactat agctagagtc ctgggcgaac aaacgatgct cgccttccag
      661 aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc gccgcgacgg
      721 ccgaggtcta ccgatctcct gaagcaaggg cagatccgtg cacagcacct tgccgtagaa
      781 gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc
      841 cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc
      901 gtggaaacgg atgaaggcac aaacccagtt gacataagcc tgttcggttc gtaaactgta
      961 atgcaagtag ccgtaatgc
//
