LOCUS       sequence-158387-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG1e - Step1 vector e (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG1e
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(368..401)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(447..466)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    491..539
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     mobile_element  816..981
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
       61 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
      121 ttctacggca aggtgctgtg cacggatctg cccttgcttc aggagatcgg tagacctcgg
      181 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
      241 ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta
      301 cagctttgtt tgtactatca acaggttgaa ctgctgatca acagatcctc tacgcggccg
      361 cggtaccata acttcgtata gcatacatta tacgaagtta tctgggttta aacggttcac
      421 atagcttagt ttttatcgag gctaactgaa acacggaagg agacaatacc ggaaggaacc
      481 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
      541 taaacgcggg gttcggtccc agggctggca ctctgtcaag tgaacgctct atggtctaaa
      601 gatttaaatc tatatctcaa tcggggttac gtcgataccc caccgagacc ccattgggac
      661 caatacgccc gcgtttcttc cttttcccca ccccaacccc caagttcggg tgaaggccca
      721 gggctcgcag ccaacgtcgg ggcggcaagc cctgccatag ccactacggg ttaattaaaa
      781 acactgacat tgacttggtt taaacaggag atccgaacca gataagtgaa atctagttcc
      841 aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc
      901 tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc
      961 caactatttt gtccgcccac agcggggcat ttttcttcct gt
//
