LOCUS       sequence-158393-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG2ab - Step2 vector ab (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG2ab
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2..35)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     mobile_element  113..278
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        663..765
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(832..851)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cataacttcg tatagcatac attatacgaa gttatctggc ctaggttaat taaacgctct
       61 atggtctaaa gtttaaactg gatactattg cacgtttcaa tcaggagatc cgaaccagat
      121 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
      181 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
      241 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt
      301 atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
      361 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
      421 ctgaatatca acgcttattt gcagcctgaa ctgaacagga gggacagctg atagaaacag
      481 aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
      541 cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata
      601 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
      661 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
      721 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      781 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      841 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      901 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      961 ctgatgaatg
//
