LOCUS       sequence-158397-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA
  TITLE     Trade-offs between enzyme fitness and solubility illuminated by deep
            mutational scanning.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114.
  PUBMED    28196882
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114."
COMMENT     Sequence Label: pSALECTNK-TEM1(S70A,D179G)/csTEM1
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(153..170)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag
       61 gctctccccg tggaggtaat aattgacgat atgatccttt ttttctgatc aaaaagtgct
      121 catcattgga aaaatcttag ctgaggttat taatggtgat ggtgatggtg ccagtgtttg
      181 atcaggctcg cgccaatttc cgcaatttga cggttacgct cgtccatggt cgcttggcta
      241 ccggtggtat aaatcacgac aatgcggctc ggtttgccgt ccggacccag cgccgcgata
      301 atgccacggc taccgcgttc gcccgcgccg cttttgtccg cgatgaacca acccgctggc
      361 agcgcgctac gcagcagcgg gcccgccact ttgtcggctt ccatccaatc aatcagctgc
      421 tggcggctcg ccagggtcag cagctcaccg gtcagcagtt tacgcagggt ggtcgccatc
      481 gccgccggca tggtcgtatc gcgttcatca ttcggaatcg cctcgttcag ttctggctcc
      541 cagcggtcca gacgggtgac gtggtcaccc atattatgca ggaacgccgt cagttctttt
      601 gggccgccaa tggtggtcag cagcagattc gcggcggtat tgtcgctcat cgtaatcgcc
      661 gcgctacaca gctcacggac ggtcataccg tcggtcaggt gtttctcggt taccgggcta
      721 tattccacca gatcgttttg gctgtaatga atacgacggc ccagctgttc ctgacccgca
      781 tccacgcggc tcaggacggc accgcacagc agcactttga aggtagacat catcgggaag
      841 cgctcctccg gacggaagct t
//
