LOCUS       sequence-158398-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA
  TITLE     Trade-offs between enzyme fitness and solubility illuminated by deep
            mutational scanning.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114.
  PUBMED    28196882
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114."
COMMENT     Sequence Label: pSALECTNK-TEM1(S70A,D179G)/csTEM1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             248..626
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(282..301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatctcgaca tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa
       61 ctcggcggct taaccgtcgc cgggatgctg gggccgtcat tgctaacgcc gcgacgtgcg
      121 actgcgcata tgcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      181 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      241 cccgaagaac gttttccaat gatggccact tttaaagttc tgctatgtgg cgcggtatta
      301 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      361 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      421 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
      481 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
      541 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tggcaccacg
      601 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
      661 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
      721 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagcagg tgagcgtggg
      781 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
      841 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
      901 gcctcactga t
//
