LOCUS       sequence-158400-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG2abc - Step2 vector abc (biGBac system for expression of protein
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG2abc
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     934..953
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
       61 cccattcgcc atcaggctct gggaggcaga ataaatgatc atatcgtcaa ttattacctc
      121 cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct
      181 tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg
      241 ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta
      301 tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc ttaaaaaaat
      361 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
      421 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
      481 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
      541 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
      601 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
      661 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
      721 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
      781 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga
      841 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
      901 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
      961 tctttacgat gccattggga tatatcaacg gtggtatatc cagtga
//
