LOCUS       sequence-158407-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSALECT plasmid with levoglucosan kinase fused to TEM-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA
  TITLE     Trade-offs between enzyme fitness and solubility illuminated by deep
            mutational scanning.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114.
  PUBMED    28196882
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114."
COMMENT     Sequence Label: pSALECTNK-LGK/csTEM1
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(155..172)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc
       61 aggctctccc cgtggaggta ataattgacg atatgatcct ttttttctga tcaaaaagtg
      121 ctcatcattg gaaaaatctt agctgaggtt attaatggtg atggtgatgg tgccagtgtt
      181 tgatcaggct cgcgccaatt tccgcaattt gacggttacg ctcgtccatg gtcgcttggc
      241 taccggtggt ataaatcacg acaatgcggc tcggtttgcc gtccggaccc agcgccgcga
      301 taatgccacg gctaccgcgt tcgcccgcgc cgcttttgtc cgcgatgaac caacccgctg
      361 gcagcgcgct acgcagcagc gggcccgcca ctttgtcggc ttccatccaa tcaatcagct
      421 gctggcggct cgccagggtc agcagctcac cggtcagcag tttacgcagg gtggtcgcca
      481 tcgccgccgg catggtcgta tcgcgttcat cattcggaat cgcctcgttc agttctggct
      541 cccagcggtc cagacgggtg acgtggtcac ccatattatg caggaacgcc gtcagttctt
      601 ttgggccgcc aatggtggtc agcagcagat tcgcggcggt attgtcgctc atcgtaatcg
      661 ccgcgctaca cagctcacgg acggtcatac cgtcggtcag gtgtttctcg gttaccgggc
      721 tatattccac cagatcgttt tggctgtaat gaatacgacg gcccagctgt tcctgacccg
      781 catccacgcg gctcaggacg gcaccgcaca gcagcacttt gaaggtagac atcatcggg
//
