LOCUS       sequence-158411-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG2abcd - Step2 vector abcd (biGBac system for expression of 
            protein complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG2abcd
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     928..947
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
       61 cgccatcagg ctctgggagg cagaataaat gatcatatcg tcaattatta cctccacggg
      121 gagagcctga gcaaactggc ctcaggcatt tgagaagcac acggtcacac tgcttccggt
      181 agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga
      241 accgacgacc gggtcgaatt tgctttcgaa tttctgccat tcatccgctt attatcactt
      301 attcaggcgt agcaaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc
      361 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
      421 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
      481 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
      541 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
      601 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
      661 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
      721 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
      781 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
      841 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      901 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      961 cgatgccatt gggatatatc aacggtggta tatccagtga tttttt
//
