LOCUS       sequence-158413-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG2abcde - Step2 vector abcde (biGBac system for expression of 
            protein complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG2abcde
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(5..38)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     mobile_element  116..281
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        666..768
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(835..854)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 taccataact tcgtatagca tacattatac gaagttatct ggcctaggtt aattaaacgc
       61 tctatggtct aaagtttaaa cactgacatt gacttggttt caatcaggag atccgaacca
      121 gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga
      181 cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca
      241 tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat ttttcttcct
      301 gttatgtttt taatcaaaca tcctgccaac tccatgtgac aaaccgtcat cttcggctac
      361 tttttctctg tcacagaatg aaaatttttc tgtcatctct tcgttattaa tgtttgtaat
      421 tgactgaata tcaacgctta tttgcagcct gaactgaaca ggagggacag ctgatagaaa
      481 cagaagccac tggagcacct caaaaacacc atcatacact aaatcagtaa gttggcagca
      541 tcacccgacg cactttgcgc cgaataaata cctgtgacgg aagatcactt cgcagaataa
      601 ataaatcctg gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga
      661 gacgttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
      721 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      781 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      841 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      901 ttaaagaccg taaagaaaaa
//
