LOCUS       sequence-158414-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-SpyTag-PuroR
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..99
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             109..162
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(797..816)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     851..870
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 gcccacatcg tgatggtgga cgcctacaag cccaccaagt ccggaggaga gggcagagga
      121 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caatgactga atacaagcct
      181 actgtcaggt tggctacaag agacgacgtt cctagagccg tgagaactct ggctgcagcc
      241 ttcgccgact accccgccac gagacacacc gttgacccag atcggcatat tgagagagtg
      301 actgaactgc aggagctgtt tcttacaaga gttggcctcg acataggcaa ggtgtgggtg
      361 gcggacgacg gcgccgccgt ggccgtctgg accactcccg aatcagttga ggctggcgcc
      421 gtattcgctg agatcggccc gagaatggct gagctcagcg ggagtaggct cgcggcacag
      481 cagcaaatgg agggactgct ggcaccacac aggcccaaag aacccgcctg gttcctggca
      541 accgtcggtg tatctcccga tcatcagggg aaaggtctgg gctctgccgt agtgctccct
      601 ggcgtggagg cagctgagag agcaggagta cctgccttct tggagacctc cgctccaagg
      661 aatcttccct tctatgaacg gttgggcttc accgtgacag ccgacgtgga agtccccgaa
      721 ggcccccgca cttggtgcat gacgaggaag cctggagcgt gagggcccgc ttcgagcaga
      781 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      841 ctttatttgt gaaatttgtg atgctattgc tttattt
//
