LOCUS       sequence-158416-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-StrepTag II-PuroR
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..34
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             44..97
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(732..751)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agggggatcc tggagccacc cccagttcga gaagtccgga ggagagggca gaggaagtct
       61 gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg actgaataca agcctactgt
      121 caggttggct acaagagacg acgttcctag agccgtgaga actctggctg cagccttcgc
      181 cgactacccc gccacgagac acaccgttga cccagatcgg catattgaga gagtgactga
      241 actgcaggag ctgtttctta caagagttgg cctcgacata ggcaaggtgt gggtggcgga
      301 cgacggcgcc gccgtggccg tctggaccac tcccgaatca gttgaggctg gcgccgtatt
      361 cgctgagatc ggcccgagaa tggctgagct cagcgggagt aggctcgcgg cacagcagca
      421 aatggaggga ctgctggcac cacacaggcc caaagaaccc gcctggttcc tggcaaccgt
      481 cggtgtatct cccgatcatc aggggaaagg tctgggctct gccgtagtgc tccctggcgt
      541 ggaggcagct gagagagcag gagtacctgc cttcttggag acctccgctc caaggaatct
      601 tcccttctat gaacggttgg gcttcaccgt gacagccgac gtggaagtcc ccgaaggccc
      661 ccgcacttgg tgcatgacga ggaagcctgg agcgtgaggg cccgcttcga gcagacatga
      721 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      781 tttgtgaaa
//
