LOCUS       sequence-158418-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-StrepTag II-PuroR
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(610..663)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(673..696)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    760..851
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(792..811)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    865..913
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 agcgggccct cacgctccag gcttcctcgt catgcaccaa gtgcgggggc cttcggggac
       61 ttccacgtcg gctgtcacgg tgaagcccaa ccgttcatag aagggaagat tccttggagc
      121 ggaggtctcc aagaaggcag gtactcctgc tctctcagct gcctccacgc cagggagcac
      181 tacggcagag cccagacctt tcccctgatg atcgggagat acaccgacgg ttgccaggaa
      241 ccaggcgggt tctttgggcc tgtgtggtgc cagcagtccc tccatttgct gctgtgccgc
      301 gagcctactc ccgctgagct cagccattct cgggccgatc tcagcgaata cggcgccagc
      361 ctcaactgat tcgggagtgg tccagacggc cacggcggcg ccgtcgtccg ccacccacac
      421 cttgcctatg tcgaggccaa ctcttgtaag aaacagctcc tgcagttcag tcactctctc
      481 aatatgccga tctgggtcaa cggtgtgtct cgtggcgggg tagtcggcga aggctgcagc
      541 cagagttctc acggctctag gaacgtcgtc tcttgtagcc aacctgacag taggcttgta
      601 ttcagtcatt gggccaggat tctcctcgac gtcaccgcat gttagcagac ttcctctgcc
      661 ctctcctccg gacttctcga actgggggtg gctccaggat ccccctccac tgccaccaga
      721 ccccccgctt tttgggtact ggcccgctag ctatcattaa gagaaatgtt ctggcacctg
      781 cacttgcact ggggacagcc tattttgcta gtttgttttg tttcgttttg ttttgatgga
      841 gagcgtatgt tagtactatc gattcacaca aaaaaccaac acacagatgt aatgaaaata
      901 aagatatttt attgcggcct gtccaatacc tcccgta
//
