LOCUS       sequence-158428-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mWasabi-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(129..839)
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
     regulatory      836..845
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      121 gcggccgctt acttgtacag ctcgtccatg ccgtcggtgg agttgcgggc cacggcgatc
      181 tcgtaaacgg tcaccttgtt gtagtccttg tcgtggttca ggatctcgat gcggtggtcc
      241 acaaagtgat agtcgggcag cttcaccgcc ttcttggccc tgtagatggt cttgaagtca
      301 acgcggtggt ggccgccgcc ctccagcagc agcttcatct tgacgtcgcc cttcagcacg
      361 ccgtcgcgca cgtacatcct ctcggtggag gcgtcccagc cggtggtctc cttctgcatc
      421 acggggccgt tgggggggaa gttctcgccc ttgaggtgta tctcgtagat gaaggagtcc
      481 tcctccatgg agatgtcgga cttcaccttc acgatgccct tgtcctcgaa ggtcatggtg
      541 cgctcccaag agtagccctc ggggaaggac tgcttgaagt agttggggat gtcgtcgggg
      601 tacttggtga aggccctgtt gccgtaactg aacgcggtgg tcagaatgtc gtaggagaag
      661 ggcagggggg ctccctcctt cacctccagg ttgatggtgt tggtgccgtc gtagggcttg
      721 ccctcgccct cgccctcgat cacgaaggcg tggccattca cgttgccctc catcttcagc
      781 ttgatcttca tgtcgggctt gattacgccc attgtggtct cctcgccctt gctcaccatg
      841 gtggcgaccg gtggatcccg ggcccgcggt accgtcgact gc
//
