LOCUS       sequence-158429-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9(H840A) in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS415_pGal-nCas9(H840A)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     420..443
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     464..480
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     464..480
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctcactaaag ggaacaaaag ctggagctcg tgacagccct ccgaaggaag actctcctcc
       61 gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg
      121 ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc
      181 agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata
      241 atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg
      301 atctattaac ggatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac
      361 attttcggtt tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta
      421 atatacctct atactttaac gtcaaggaga aaaaaccccg gattctagaa ctagtggatc
      481 ccccgggaaa aaaatggaca agaagtactc cattgggctc gatatcggca caaacagcgt
      541 cggttgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg
      601 caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg
      661 ggagacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa
      721 gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc
      781 tttcttccat aggctggagg
//
