LOCUS       sequence-158430-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9(H840A) in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS415_pGal-nCas9(H840A)
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(108..119)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cttagtcaga ttatcgaact tccgttgtgt gatcagtttg gcgttcagca gctgccgcca
       61 ataatttttc attttcttga caacttcttc tgaggggacg ttatcactct tccctctatt
      121 tttatcggat cttgtcaaca ctttattatc aatagaatca tctttgagaa aagactgggg
      181 cacgatagca tccacgtcgt agtcggagag ccgattgatg tccagttcct gatccacgta
      241 catgtccctg ccgttctgca ggtagtacag gtagagcttc tcattctgaa gctgggtgtt
      301 ttcaactggg tgttccttaa ggatttggga ccccagttct tttataccct cttcaatcct
      361 cttcatcctt tccctactgt tcttctgtcc cttctgggta gtttggttct ctcgggccat
      421 ctcgataacg atattctcgg gcttatgcct tcccattact ttgacgagtt catccacgac
      481 cttaacggtc tgcagtattc cctttttgat agctgggcta cctgcaagat tagcgatgtg
      541 ctcgtgaaga ctgtccccct ggccagaaac ttgtgctttc tggatgtcct ccttaaaggt
      601 gagagagtca tcatggatca actgcatgaa gttccggttg gcaaatccat cggacttaag
      661 aaaatccagg attgtctttc cactctgctt gtctcggatc ccattgatca gttttcttga
      721 cagccgcccc catcctgtat atcggcgcct cttgagctgt ttcatgactt
//
