LOCUS       sequence-158431-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             100..120
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gaaatggcac cgaagaagaa gcgtaaagtc
      121 ggaatccacg gagttcctgc ggcaatggac aagaagtact ccattgggct cgctatcggc
      181 acaaacagcg tcggttgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc
      241 aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg
      301 ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat
      361 acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag
      421 gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag
      481 cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac
      541 ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg
      601 ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg
      661 gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac
      721 aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg
      781 agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggg
//
