LOCUS       sequence-158446-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains aTc inducible Scaffold-mCherry-AAV and TetR genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH001
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(354..371)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(433..451)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        529..584
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    530..548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(676..695)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
       61 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tgctgccgct gccactaccg
      121 gatcccaggg ggccgtagcg ggtttcatat ttgctaacaa tattctcgca gcggcctact
      181 tctttctcca gttcagcaat ctcagtacgc agtgcggtgt tttccttctc cagaaaagct
      241 gcccgaattg tgatagatcc accgctgccg gacccagatc cacgccttct ctttggcggt
      301 aatgcaggtg gaggctggaa gtacaggttt tcggtagtcg ggatgtcgta gtcgtgatgg
      361 tgatggtgat gcatagatct cttagtttgc gttgggccac acttcaggta ccgctagcgt
      421 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      481 tttcttagtc gactctagtg gatctgctag tcatatggat tttcactttt ctctatcact
      541 gatagggagt ggtaaaataa ctctatcaac gatagagtgt caacaaaaat taggaattaa
      601 tgatgtcaag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      661 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      721 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      781 tagataggca ccatactcac ttttgccctt tagaaggg
//
