LOCUS       sequence-158447-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains aTc inducible Scaffold-mCherry-AAV and TetR genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH001
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             311..340
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             356..373
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(429..446)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      599..645
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(687..704)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
       61 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      121 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      181 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      241 aaggcagcaa acgacgaaaa ctacgctgca gcagtttaat aataactcga gtaatgagaa
      301 gcttgggccc gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca
      361 tcatcatcat cattgagttt aaacggtctc cagcttggct gttttggcgg atgagagaag
      421 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
      481 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      541 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
      601 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      661 gaactaattt ctagactgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      721 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      781 gataacgcag gaaagaacat gtgagcaa
//
