LOCUS       sequence-158448-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTet-Scaffold with no fluorescent protein. Used as a control for 
            autofluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH002
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        59..114
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    95..113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     192..210
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             272..289
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             545..574
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             590..607
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(663..680)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 atgcgctgtt aatcacttta cttttatcta atcttgacat cattaattcc taatttttgt
       61 tgacactcta tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagtga
      121 aaatccatat gactagcaga tccactagag tcgactaaga aaccattatt atcatgacat
      181 taacctataa aaataggcgt atcacgaggc cctttcgtct tcacgctagc ggtacctgaa
      241 gtgtggccca acgcaaacta agagatctat gcatcaccat caccatcacg actacgacat
      301 cccgactacc gaaaacctgt acttccagcc tccacctgca ttaccgccaa agagaaggcg
      361 tggatctggg tccggcagcg gtggatctat cacaattcgg gcagcttttc tggagaagga
      421 aaacaccgca ctgcgtactg agattgctga actggagaaa gaagtaggcc gctgcgagaa
      481 tattgttagc aaatatgaaa cccgctacgg ccccctgaac tcgagtaatg agaagcttgg
      541 gcccgaacaa aaactcatct cagaagagga tctgaatagc gccgtcgacc atcatcatca
      601 tcatcattga gtttaaacgg tctccagctt ggctgttttg gcggatgaga gaagattttc
      661 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
      721 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
      781 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
      841 acgaa
//
