LOCUS       sequence-158451-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pAAV-CASI-Cas9C
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(108..119)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      716..725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      720..729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cttagtcaga ttatcgaact tccgttgtgt gatcagtttg gcgttcagca gctgccgcca
       61 ataatttttc attttcttga caacttcttc tgaggggacg ttatcactct tccctctatt
      121 tttatcggat cttgtcaaca ctttattatc aatagaatca tctttgagaa aagactgggg
      181 cacgatatga tccacgtcgt agtcggagag ccgattgatg tccagttcct gatccacgta
      241 catgtccctg ccgttctgca ggtagtacag gtagagcttc tcattctgaa gctgggtgtt
      301 ttcaactggg tgttccttaa ggatttggga ccccagttct tttataccct cttcaatcct
      361 cttcatcctt tccctactgt tcttctgtcc cttctgggta gtttggttct ctcgggccat
      421 ctcgataacg atattctcgg gcttatgcct tcccattact ttgacgagtt catccacgac
      481 cttaacggtc tgcagtattc cctttttgat agctgggcta cctgcaagat tagcgatgtg
      541 ctcgtgaaga ctgtccccct ggccagagtt atgcgcaata atatcgttcg ccacaaagtt
      601 atgcgggccc ggcacggtca gatcaaacac ttcttccacg ccatccggtt caatgctcac
      661 aatcggatcc caatacacat cgctctgcgc cagctgacgc agttccgggc acgccgccgc
      721 catggtggcg gcgctagcct gttcgtcacc caggacctgt agaaaaaaaa agaaaacatg
      781 aacatggtta gtagagg
//
