LOCUS       sequence-158453-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pAAV-CASI-Cas9C
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..63)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ataaatttcc tttattagcc aaccggttca tcgagctcga gacaccttcc tcttcttctt
       61 ggggtcagcc ctgctgtctc caccgagctg agagaggtcg attcttgttt catagagccc
      121 cgtaattgac tgatgaatca gtgtggcgtc caggacctcc tttgtagagg tgtaccgctt
      181 tctgtctatg gtggtgtcga agtacttgaa ggctgcaggc gcgcccaagt tggtcagagt
      241 aaacaagtgg ataatgtttt ctgcctgctc cctgatgggc ttatccctgt gcttattgta
      301 agcagaaagc accttatcga ggttagcgtc ggcgaggatc actcttttgg agaattcgct
      361 tatttgctcg atgatctcat caaggtagtg tttgtgttgt tccacgaaca gctgcttctg
      421 ctcattatct tcgggagacc ctttgagctt ttcatagtgg ctggccagat acaagaaatt
      481 aacgtattta gagggcagtg ccagctcgtt acctttctgc agctcgcccg cactagcgag
      541 cattcgtttc cggccgtttt caagctcaaa gagagagtac ttgggaagct taatgatgag
      601 gtcttttttg acctctttat atcctttcgc ctcgagaaag tcgatggggt ttttttcgaa
      661 gcttgatcgc tccatgattg tgatgcccag cagttccttg acgcttttga gttttttaga
      721 cttccctttc tccactttgg ccacaaccag tacactgtaa gcgactgtag gagaatcgaa
      781 tccgccgtat ttcttggggt cccaatcttt
//
