LOCUS       sequence-158454-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9C-VPR in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pAAV-CMV-Cas9C-VPR
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             633..644
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gaagccgcta gcgctaccgg tcgccaccat ggcggcggcg tgcccggaac tgcgtcagct
       61 ggcgcagagc gatgtgtatt gggatccgat tgtgagcatt gaaccggatg gcgtggaaga
      121 agtgtttgat ctgaccgtgc cgggcccgca taactttgtg gcgaacgata ttattgcgca
      181 taactctggc cagggggaca gtcttcacga gcacatcgct aatcttgcag gtagcccagc
      241 tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat gaactcgtca aagtaatggg
      301 aaggcataag cccgagaata tcgttatcga gatggcccga gagaaccaaa ctacccagaa
      361 gggacagaag aacagtaggg aaaggatgaa gaggattgaa gagggtataa aagaactggg
      421 gtcccaaatc cttaaggaac acccagttga aaacacccag cttcagaatg agaagctcta
      481 cctgtactac ctgcagaacg gcagggacat gtacgtggat caggaactgg acatcaatcg
      541 gctctccgac tacgacgtgg atcatatcgt gccccagtct tttctcaaag atgattctat
      601 tgataataaa gtgttgacaa gatccgataa aaatagaggg aagagtgata acgtcccctc
      661 agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag ctgctgaacg ccaaactgat
      721 cacacaacgg aagttcgata atctgactaa ggctgaacga ggtggcctgt ctgagttgga
      781 taaagccggc ttcatcaaaa ggcagcttgt tgagacacgc cagatcacca agcacgtggc
      841 cc
//
