LOCUS       sequence-158456-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that expresses Cas9N in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pSMVP-Cas9N
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggattctcct aggcagagcc aggtaatctc caggctggag ttcatcgact cgtttccagc
       61 cctgaggtgt gagaaatcgg tggttggcag tggccctaat ggatctcccc agtctagtgg
      121 tgaggcggta cacaggtttg atgccggtac agaaagctct actcactcta gccctctcca
      181 ggcggtaagt ctgaggattc agagcccaca cggaaaaatt ctgctgtgac accagttctc
      241 taataggcac tcgtcgtcca tcggccaggg taatgagagt atcgccagcc agacaaactt
      301 gtgctttctg gatgtcctcc ttaaaggtga gagagtcatc atggatcaac tgcatgaagt
      361 tccggttggc aaatccatcg gacttaagaa aatccaggat tgtctttcca ctctgcttgt
      421 ctcggatccc attgatcagt tttcttgaca gccgccccca tcctgtatat cggcgcctct
      481 tgagctgttt catgactttg tcgtcgaaga gatgagcgta agttttcaag cgttcttcaa
      541 tcatctccct atcttcaaac aacgtaaggg tgaggacaat gtcctcaaga atgtcctcgt
      601 tctcctcatt gtccaggaag tccttgtctt taatgatttt caggagatcg tgatacgttc
      661 ccagggatgc gttgaagcga tcctccactc cgctgatttc aacagagtcg aaacattcaa
      721 tcttt
//
